Systems and Methods of Analyzing Two Dimensional Gels
First Claim
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1. A method for analyzing a 2-dimensional (2D) gel, the method comprising:
- receiving a first image of a gel based on a first protein sample labeled with a first fluorophore;
receiving a second image of the gel based on a second protein sample labeled with a second fluorophore;
applying linear normalization to image intensity values of the second image based on the first image to provide a linear normalized image; and
comparing image intensity values of the linear normalized image with image intensity values of the first image to provide a compared image.
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Abstract
Systems and methods of analyzing two dimensional gels are provided, in one embodiment, a method is provided for analyzing a 2-dimensiαnal gel. The method comprises receiving a first image of a gel based on a first protein sample labeled with a first fluorophore, receiving a second image of the gel based on a second protein sample labeled with a second fluorophore, applying linear normalization to image intensity values of the second image to provide a linear normalized image, and comparing image intensity values of the linear normalized image from image intensity values of the first image to provide a compared image.
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Citations
29 Claims
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1. A method for analyzing a 2-dimensional (2D) gel, the method comprising:
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receiving a first image of a gel based on a first protein sample labeled with a first fluorophore; receiving a second image of the gel based on a second protein sample labeled with a second fluorophore; applying linear normalization to image intensity values of the second image based on the first image to provide a linear normalized image; and comparing image intensity values of the linear normalized image with image intensity values of the first image to provide a compared image. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A computer readable medium having computer executable instructions for performing the method comprising:
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receiving a first image of a 2-D differential gel based on a first protein sample labeled with a first fluorophore; receiving a second image of the 2-D differential gel based on a second protein sample labeled with a second fluorophore; applying linear normalization to image intensity values of the second image based on the first Image to provide a linear normalized image; performing a pixel by pixel subtraction of image intensity values of the linear normalized image and image intensity values of the first image to provide a differential image; determining a second numerical derivative on image intensity values of the differential image to determine protein spot centers; and performing a non-linear fitting on image intensity values of the differential image based on the determined protein spot centers to determine spot intensity volumes of protein spots on the differential image. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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23. A system for analyzing a 2-dimensional (2D) gel, the system comprising:
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an image normalization and compare module that applies linear normalization to one of a first image of a gel based on a first protein sample labeled with a first fluorophore and a second image of the gel based on a second protein sample labeled with a second fluorophore based on the other of the first and second image and generates a compared image that is a comparison of a normalized one of the first image and second image to a non-normalized one of the first image and second image; and a spot detection and fitting component that performs a non-linear fitting on image intensity values of the compared image based on determined protein spot centers to determine spot intensity volumes of protein spots on the compared image. - View Dependent Claims (24, 25, 26, 27, 28, 29)
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Specification