PROTECTING ALGAE FROM BODY FLUIDS

• US 20100047311A1
• Filed: 11/22/2007
• Published: 02/25/2010
• Est. Priority Date: 11/22/2006
• Status: Abandoned Application

• Current Assignee: Beta Cell Technologies Pty., Ltd.
• Original Assignee: Beta Cell Technologies Pty., Ltd.
• Inventors: Evron, Yoav, Schneider, Chanan, Rotem, Avi, Neufeld, Tova
• Application Number:
  US12/515,818
• Publication Number:
  US 20100047311A1
• Time in Patent Office: Days
• US Class Current: 424/423
• CPC Class Codes: A61F 2/022 Artificial gland structures using bioreactors hemodialysis A61M1/16; hemofiltration A61M1/34; other extracorporeal blood treatment A61M1/36
• CPC Class Codes: A61K 35/39 Pancreas; Islets of Langerhans Langerhans cells of epidermis A61K35/36
• CPC Class Codes: A61L 27/38 containing added animal cells organs or tissue containing native cells A61L27/36

Abstract

Apparatus (20) is provided for implantation into a body of a subject, including isolated functional cells (28). At least one first barrier (22) having a first molecular weight cutoff is disposed with respect to the functional cells so as to protect the functional cells from components disposed within body fluid of the subject having molecular weights higher than the first cutoff. Photosynthetic elements (26) are disposed with respect to the functional cells so as to provide oxygen thereto. At least one second barrier (24) has a second molecular weight cutoff that is lower than the first cutoff. The second barrier is disposed with respect to the photosynthetic elements so as to protect the photosynthetic elements from components disposed within the body fluid of the subject having molecular weights higher than the second cutoff. Other embodiments are also described.

Claims

1. Apparatus for implantation into a body of a subject, comprising:

isolated functional cells;

at least one first semi-permeable barrier having associated therewith a first molecular weight cutoff, disposed with respect to the functional cells so as to protect the functional cells from components disposed within a body fluid of the subject having molecular weights higher than the first cutoff;

photosynthetic elements, disposed with respect to the functional cells so as to provide oxygen thereto; and

at least one second semi-permeable barrier having associated therewith a second molecular weight cutoff that is lower than the first cutoff, the second barrier disposed with respect to the photosynthetic elements so as to protect the photosynthetic elements from components disposed within the body fluid of the subject having molecular weights higher than the second cutoff.

2. The apparatus according to claim 1, wherein the components disposed within the body fluid include antibiotic molecules, and wherein the second semi-permeable barrier is configured to protect the photosynthetic elements from the antibiotic molecules.

3. The apparatus according to claim 1, wherein the photosynthetic elements comprise algae.

4. The apparatus according to claim 1, wherein the photosynthetic elements comprise at least one photosynthetic element selected from the group consisting of:

isolated chloroplasts, and photosynthetic organisms.

5. The apparatus according to claim 1, wherein the apparatus is configured for subcutaneous implantation in the subject.

6. The apparatus according to claim 1, further comprising a light source configured to provide light for the photosynthetic elements.

7. The apparatus according to claim 6, wherein the light source comprises a light emitting diode (LED).

8. The apparatus according to claim 1, wherein the first cutoff is greater than 1000 Daltons.

9. The apparatus according to claim 1, wherein the second cutoff is greater than 100 Daltons.

10. The apparatus according to claim 1, wherein the second cutoff is greater than 300 Daltons.

11. The apparatus according to claim 1, wherein the second cutoff is less than 5000 Daltons.

12. The apparatus according to claim 1, wherein the second cutoff is less than 1500 Daltons.

13. The apparatus according to claim 1, wherein the first cutoff is greater than two times the second cutoff.

14. The apparatus according to claim 1, wherein the first cutoff is greater than 10 times the second cutoff.

15. The apparatus according to claim 1, wherein the functional cells produce a desired large molecule having a molecular weight associated therewith, and wherein the molecular weight cutoff of the second barrier is lower than the molecular weight of the large molecule.

16. The apparatus according to claim 1, wherein the functional cells are capable of performing at least one of the actions selected from the list consisting of:

absorbing a substance from the body, and degrading a substance from the body, and wherein the molecular weight cutoff of the second barrier is lower than a molecular weight of the substance.

17. The apparatus according to claim 1, wherein the second cutoff is between 200 and 1000 Daltons.

18. The apparatus according to claim 1, wherein the second cutoff is between 300 and 500 Daltons.

19. The apparatus according to claim 1, wherein the at least one second semi-permeable barrier comprises a hydrophobic semi-permeable barrier.

20. The apparatus according to claim 1, further comprising a housing, wherein the first semi-permeable barrier and the second semi-permeable barrier are coupled to the housing.

21. The apparatus according to claim 20, wherein the first semi-permeable barrier is shaped to define the housing.

22. The apparatus according to claim 20, wherein the first semi-permeable barrier and the second semi-permeable barrier are disposed within the housing.

23. The apparatus according to claim 1, wherein the first semi-permeable barrier surrounds a first region of the apparatus, and wherein the second semi-permeable barrier surrounds a second region of the apparatus.

24. The apparatus according to claim 23, wherein the first and second barriers are gas permeable.

25. The apparatus according to claim 24, wherein the first and second barriers are configured to facilitate bidirectional passage therethrough of gases between the first and second regions.

26. The apparatus according to claim 1, wherein the at least one first semi-permeable barrier comprises a plurality of first semi-permeable barriers surrounding respective portions of the functional cells, and wherein the at least one second semi-permeable barrier comprises a plurality of second semi-permeable barriers surrounding respective portions of the photosynthetic elements.

27. The apparatus according to claim 26, wherein the molecular weight cutoffs of each of the first barriers is greater than two times the molecular weight cutoffs of each of the second barriers.

28. The apparatus according to claim 26, wherein the molecular weight cutoffs of each of the first barriers is greater than ten times the molecular weight cutoffs of each of the second barriers.

29. The apparatus according to claim 26, wherein the plurality of first semi-permeable barriers are generally spherical.

30. The apparatus according to claim 26, wherein the plurality of second semi-permeable barriers are generally spherical.

31. The apparatus according to claim 26, wherein a majority of the first semi-permeable barriers are in contact with at least one other one of the first or second barriers.

32. The apparatus according to claim 26, wherein a majority of the first semi-permeable barriers are not in contact with at least one other one of the first or second barriers.

33. A method, comprising:

protecting isolated functional cells from a first set of components disposed within body fluid of a subject by using at least one first semi-permeable barrier having associated therewith a first molecular weight cutoff;

protecting photosynthetic elements from a second set of components disposed within the body fluid of the subject by using at least one second semi-permeable barrier having associated therewith a second molecular weight cutoff, the first molecular weight cutoff being lower than the first cutoff; and

implanting within the body of the subject the first semi-permeable barrier, the second semi-permeable barrier, the functional cells, and the photosynthetic elements.

34. The method according to claim 33, wherein implanting comprises subcutaneously implanting the first semi-permeable barrier, the second semi-permeable barrier, the functional cells, and the photosynthetic elements.

35. The method according to claim 33, wherein the molecular weight cutoff of the first barrier is at least 1000 Daltons, and wherein providing the first barrier comprises providing the first barrier having the cutoff of at least 1000 Daltons.

36. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is at least 100 Daltons, and wherein providing the second barrier comprises providing the second barrier having the cutoff of at least 100 Daltons.

37. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is at least 300 Daltons, and wherein providing the second barrier comprises providing the second barrier having the cutoff of at least 300 Daltons.

38. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is less than 1500 Daltons, and wherein providing the second barrier comprises providing the second barrier having the cutoff of less than 1500 Daltons.

39. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is less than 5000 Daltons, and wherein providing the second barrier comprises providing the second barrier having the cutoff of less than 5000 Daltons.

40. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is between 200 and 1000 Daltons, and wherein providing the second barrier comprises providing the second barrier having the cutoff of between 200 and 1000 Daltons.

41. The method according to claim 33, wherein the molecular weight cutoff of the second barrier is between 300 and 500 Daltons, and wherein the second barrier comprises providing the second barrier having the cutoff of between 300 and 500 Daltons.

42. The method according to claim 33, wherein the molecular weight cutoff of the first barrier is greater than two times the molecular weight cutoff of the second barrier, and wherein providing the first barrier comprises providing the first barrier having the cutoff greater than two times the cutoff of the second barrier.

43. The method according to claim 33, wherein the molecular weight cutoff of the first barrier is greater than ten times the molecular weight cutoff of the second barrier, and wherein providing the first barrier comprises providing the first barrier having the cutoff greater than ten times the cutoff of the second barrier.

44. The method according to claim 33, further comprising facilitating bidirectional passage of gases between the isolated functional cells and the photosynthetic elements.

45. The method according to claim 33, wherein protecting the photosynthetic elements comprises restricting passage through the second semi-permeable barrier of a portion of the body fluid that has passed through the first semi-permeable barrier.

46. The method according to claim 33, wherein:

protecting the isolated functional cells comprises providing a plurality of first semi-permeable barriers, the plurality of first semi-permeable barriers providing protection to the isolated functional cells by surrounding respective portions thereof, and

protecting the photosynthetic elements comprises providing a plurality of second semi-permeable barriers, the plurality of second semi-permeable barriers providing protection to the photosynthetic elements by surrounding respective portions thereof.

47. The method according to claim 33, wherein protecting the photosynthetic elements comprises restricting passage, through the second barrier, of a molecule produced by the functional cells, and wherein the molecular weight cutoff of the second barrier is lower than the molecular weight of the molecules produced by the functional cells.

48. The method according to claim 33, wherein protecting the photosynthetic elements comprises restricting passage, through the second barrier, of a molecule produced by the body and absorbable or degradable by the functional cells, and wherein the molecular weight of the molecule is lower than the molecular weight cutoff of the first barrier and higher than the molecular weigh cutoff of the second barrier.