CARTRIDGE, SYSTEM AND METHOD FOR AUTOMATED MEDICAL DIAGNOSTICS

• US 20100047774A1
• Filed: 06/16/2006
• Published: 02/25/2010
• Est. Priority Date: 06/23/2005
• Status: Active Grant

• Current Assignee: Biocartis NV (Biocartis Group NV)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: De Gier, Ronald, Pross, Gerhard, Seher, Jens-Peter, Luedke, Gerd, Schaefer, Danny Genius Aldegonda, Jongerius, Michiel Johannes, Boos, Andreas, Bacher, Johannes, De Jong, Michiel, Van Haag, Chris, Van Den Bijgaart, Adrianus Wilhelmus Dionisius Maria
• Granted Patent: US 9,568,424 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/028 Modular arrangements
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/02 Identification, exchange or storage of information
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/1805 Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/545 for laboratory containers
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 35/00069 whereby the sample substrate is of the bio-disk type, i.e. having the format of an optical disk

Abstract

Disclosed is a cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. The cartridge comprises a generic part and one or more separate application-specific parts, which are connectable to the generic part. Also disclosed is a system for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences. This system comprising a reusable apparatus, wherein said apparatus is configured to receive a cartridge and to control a process for the detection of the presence, absence and/or amount of a target nucleotide sequence in the sample, being present in said cartridge.

Claims

1. A cartridge for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences, characterized in that the cartridge comprises a generic part and one or more separate application-specific parts, which are connectable to the generic part.

2. A cartridge according to claim 1, wherein one of the one or more specific parts is a PCR body having one or more thermocycling chambers and comprising a number of primers.

3. A cartridge according to claim 2, wherein at least one primer is arranged in each one of the one or more thermocycling chambers.

4. A cartridge according to claim 2, wherein at least one of the number of primers is spotted on the PCR body.

5. A cartridge according to claim 2, wherein the PCR body is disc-shaped.

6. A cartridge according to claim 2, wherein the PCR body comprises one or more thermal masses.

7. A cartridge according to claim 1, wherein one of the one or more application-specific parts is a detection device.

8. A cartridge according to claim 2, wherein the detection device is selected on the basis of the primers in the PCR body.

9. A cartridge according to claim 1, wherein one of the one or more application-specific parts is a sample introduction device configured to prepare a sample to a specific state.

10. A cartridge according to claim 9, wherein the sample introduction device is a pre-lysis device configured to prepare a sample to a specific state.

11. A cartridge according to claim 1, wherein at least one of the one or more application-specific parts is connectable on the main body with a click-fit connection.

12. A cartridge according to claim 1, wherein each space for holding the sample or part thereof in the generic part and the one or more application-specific parts is air-tight.

13. A cartridge according to claim 1, wherein at least one of the one or more of the application-specific parts is provided with an identification device.

14. A cartridge according to claim 12, wherein each of the one or more application-specific parts and the generic part is provided with an identification device.

15. System for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences, said system comprising a reusable apparatus, wherein said apparatus is configured to receive a cartridge according to claim 1 and to control a process for the detection of the presence, absence and/or amount of a target nucleotide sequence in the sample, being present in said cartridge.

16. A method for the detection of the presence, absence and/or amount of a target nucleotide sequence in a sample comprising one or more nucleic acid sequences, wherein the method comprises the steps of:

providing a sample from an organism;

performing steps for isolation of the nucleic acid sequences from the sample;

performing steps for amplification of (part of) the nucleic acid sequences to thereby provide amplicons;

detecting the presence, absence and/or amount of the amplicons corresponding to the target nucleotide sequence amongst the nucleic acid sequences in the sample,

characterized in that the method is being performed in a cartridge according to claim 1.

17. A method according to claim 1, wherein the method further comprises the selection of one or more application-specific part and mounting said application-specific part on the generic part of the cartridge.