Sample analyser

• US 20100047790A1
• Filed: 12/21/2007
• Published: 02/25/2010
• Est. Priority Date: 12/21/2006
• Status: Abandoned Application

• Current Assignee: Oxford Gene Technology IP Limited (Sysmex Corporation)
• Original Assignee: Oxford Gene Technology IP Limited (Sysmex Corporation)
• Inventors: Southern, Edwin, Patel, Kaajal, Milner, Natalie
• Application Number:
  US12/448,345
• Publication Number:
  US 20100047790A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0822 Slides
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2400/088 by specific surface properties
• CPC Class Codes: B01L 3/0293 for liquids
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 1/312 for samples mounted on planar substrates
• CPC Class Codes: G01N 2001/282 with mapping; Identification of areas; Spatial correlated pattern

Abstract

There are provided processes for analysing a plurality of different samples. The processes comprise the steps of: a) applying the samples to a support, to which an analytical component is immobilised; and b) allowing the samples to interact with the analytical component, thus permitting analysis of the samples. The samples are applied in step a) to different areas of the support to produce a spatial arrangement of samples on the support. The spatial arrangement of the samples is maintained in step b), thus permitting the results of the analysis to be matched to individual samples.

Claims

1. A process for analysing a plurality of different samples, comprising the steps of:

a) applying the samples to a support, to which an analytical component is immobilised; and

b) allowing the samples to interact with the analytical component, thus permitting analysis of the samples,

wherein the individual samples are applied in step a) to different areas of the support to produce a spatial arrangement of samples on the support, and

wherein the spatial arrangement is maintained in step b), thus permitting the results of the analysis to be matched to individual samples.

2. A process according to claim 1, further comprising matching the results of the analysis to individual samples.

24. A process, device or kit according to any preceding claim, wherein the support is impermeable to the reagents that are applied to the device during use of the device.

25. A process, device or kit according to any of claims 1-23, wherein the support is permeable to the reagents that are applied to the device during use of the device.

26. A process, device or kit according to any preceding claim, wherein the specific binding reagent is a nucleic acid.

27. A process, device or kit according to any of claims 1 to 25, wherein the specific binding reagent is an antibody or antibody fragment.

28. A process, device or kit according to any of claims 1 to 25, wherein the specific binding reagent is an aptamer.

29. A process, device or kit according to any of claims 1 to 25, wherein the specific binding reagent is a small molecule.

30. A process, device or kit according to claim 29, wherein the small molecule is an organic molecule with a molecular weight of less than 2000 Daltons.

31. A process, device or kit according to claim 29, wherein the small molecule is a peptide or peptide analog comprising at least 5 amino acid residues.

32. A process, device or kit according to any of claims 1 to 25, wherein different specific binding reagents are immobilised in patches on the support.

33. A process, device or kit according to claim 32, wherein a single cell is applied to a patch of specific binding reagent.

34. A process, device or kit according to claim 32, wherein each patch is sized to permit parallel analysis of at least two samples.

35. A process, device or kit according to claim 34, wherein at least two cells are applied to a patch of specific binding reagent.

36. A process, device or kit according to any of claims 1 to 25, wherein each individual sample comprises a single cell.

3. A process for analysing a plurality of different individual cells, comprising the steps of:

a) applying material derived from individual cells to a support, to which a specific binding reagent is immobilised; and

b) allowing the material to interact with the specific binding reagent, thus permitting analysis of the material,

wherein the material derived from different individual cells is applied in step a) to different areas of the support to produce a spatial arrangement of material on the support, and wherein the spatial arrangement is maintained in step b), thus permitting the results of the analysis to be matched to individual cells.

4. A process according to claim 3, further comprising matching the results of the analysis to individual cells.

5. A process according to claim 3, wherein step a) comprises:

(i) applying cells to the support;

then

(ii) releasing material from the cells.

6. A process according to claim 3, wherein step a) comprises:

(i) releasing material from the cells;

then

(ii) applying the released material to the support.

7. A process according to claim 6, wherein step (i) comprises releasing material from the cells onto different areas of a substrate to produce a spatial arrangement of material on the substrate, and step (ii) comprises transferring target analytes from the substrate to the support, whilst maintaining the spatial arrangement generated in step (i).

8. A process according to claim 7, wherein the substrate is impermeable to cells or cell components but permeable to target analytes and transfer reagents.

9. A device for analysing a plurality of different individual cells, comprising a support, to which a specific binding reagent is immobilised, and on which support material derived from a plurality of different individual cells is located in a spatial arrangement that permits the results of analysis using the analytical component to be matched to individual cells.

10. A device for analysing a plurality of different individual cells, comprising:

(i) a support, to which a specific binding reagent is immobilised; and

(ii) a transfer substrate permeable to lysis reagents and target analytes but impermeable to cells or cell components,

wherein the transfer substrate is positioned against or in close proximity to the support.

11. A kit for analysing a plurality of different individual cells, comprising:

(i) a support, to which a specific binding reagent is immobilised; and

(ii) a material applicator, for applying material derived from a plurality of different individual cells to the support in a spatial arrangement that permits the results of analysis using the analytical component to be matched to individual cells.

12. The kit of claim 11, wherein the material applicator is an applicator for applying the cells to the support and then releasing material from the cells.

13. The kit of claim 11, wherein the material applicator is an applicator for releasing material from the cells and then applying the released material to the support.

14. The kit of claim 13, wherein the material applicator comprises a substrate permeable to lysis reagents and target analytes but impermeable to cells or cell components.

15. A process for analysing a plurality of different samples, comprising the steps of:

a) applying the samples to different areas of a transfer substrate to produce a spatial arrangement of samples on the transfer substrate;

then

b) transferring target analytes from the transfer substrate to a support, to which a specific binding reagent is immobilised; and

c) allowing the target analytes to interact with the specific binding reagent, thus permitting analysis of the samples,

wherein the spatial arrangement of the target analytes is maintained in steps b) and c), thus permitting the results of the analysis to be matched to individual samples.

16. A process according to claim 15, further comprising matching the results of the analysis to individual samples.

17. A process according to claim 15, wherein the transfer substrate is permeable to target analytes and transfer reagents.

18. A process according to claim 17, wherein transfer reagents are applied to the transfer substrate in step b), thus permitting transfer of target analytes from the transfer substrate to the support.

19. A process according to any of claims 15 to 18, wherein the transfer substrate is positioned against or in close proximity to the support in step b), to facilitate transfer of target analytes from the transfer substrate to the support.

20. A device for analysing a plurality of different samples, comprising:

(i) a support, to which a specific binding reagent is immobilised; and

(ii) a transfer substrate permeable to target analytes and transfer reagents, positioned against or in close proximity to the support.

21. A device according to claim 20, wherein a plurality of different samples are located on the support in a spatial arrangement that permits the results of analysis using the analytical component to be matched to individual samples.

22. A kit for analysing a plurality of different samples, comprising:

(i) a support, to which a specific binding reagent is immobilised;

(ii) a transfer substrate permeable to target analytes and transfer reagents;

(iii) a material transferor for transferring target analytes from the substrate to the support, which permits a spatial arrangement of samples on the substrate to be maintained when target analytes are transferred to the support.

23. The kit of claim 22, wherein the material transferor is a transfer reagent.