GERM LINEAGE DERIVED FEEDER CELLS AND METHODS THEREOF
First Claim
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1. A method of generating human germ lineage derived feeder cells (GLDF cells), said method comprising:
- a. culturing human embryonic stem cells (hESC) on growth medium to obtain germ lineage cells;
b. culturing said germ lineage cells on GLDF medium to obtain fibroblast like cells; and
c. treating said fibroblast like cells to generate human GLDF cells.
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Abstract
The present disclosure relates to human germ layer derived feeder cells (GLDF cells) and method of generation thereof. Further, it relates to a method for culturing and propagating human embryonic stem cells (hESCs) in a substantially undifferentiated state for several passages on the human GLDF cells. In particular, the present disclosure relates to human GLDF cells which are capable of supporting proliferation of hESCs in a substantially undifferentiated and pluripotent state for several passages.
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Citations
25 Claims
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1. A method of generating human germ lineage derived feeder cells (GLDF cells), said method comprising:
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a. culturing human embryonic stem cells (hESC) on growth medium to obtain germ lineage cells; b. culturing said germ lineage cells on GLDF medium to obtain fibroblast like cells; and c. treating said fibroblast like cells to generate human GLDF cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification