Method for genetic identification of unknown organisms
First Claim
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1. A method of identifying pathogenic and non-pathogenic organisms in a sample, comprising:
- preparing the sample,isolating the organisms from the sample into microdroplets,amplifying the organisms,sorting said microdroplets,analyzing said microdroplets, andclassifying and identifying the organisms.
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Abstract
A method of rapid, genome and proteome based identification of unknown pathogenic or non-pathogenic organisms in a complex sample. The entire sample is analyzed by creating millions of emulsion encapsulated microdroplets, each containing a single pathogenic or non-pathogenic organism sized particle and appropriate reagents for amplification. Following amplification, the amplified product is analyzed.
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Citations
49 Claims
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1. A method of identifying pathogenic and non-pathogenic organisms in a sample, comprising:
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preparing the sample, isolating the organisms from the sample into microdroplets, amplifying the organisms, sorting said microdroplets, analyzing said microdroplets, and classifying and identifying the organisms. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 34, 35)
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22. An apparatus for identifying pathogenic and non-pathogenic organisms in a sample, comprising:
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a sample preparation system for preparing the sample, an isolating system for isolating the organisms from the sample into microdroplets, an amplifier for amplifying the organisms, a sorter for sorting said microdroplets, an analyzer for analyzing said microdroplets, and a system for classifying and identifying the organisms. - View Dependent Claims (23, 24, 25, 26)
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27. A method of identifying organisms in a sample, wherein said organisms may be pathogenic organisms or non-pathogenic organisms, comprising the steps of:
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preparing the sample containing the organisms, isolating the organisms from the sample into microdroplets, amplifying the organisms, sorting said microdroplets, analyzing said microdroplets, and classifying and identifying the organisms. - View Dependent Claims (28, 29, 30, 31, 32, 33, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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Specification