Method for Improving Yield of Cellulose Conversion Processes

• US 20100055747A1
• Filed: 11/13/2007
• Published: 03/04/2010
• Est. Priority Date: 11/13/2006
• Status: Abandoned Application

• Current Assignee: Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Danisco USA Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Mitchinson, Colin, Kelemen, Bradley, Larenas, Edmund A.
• Application Number:
  US12/514,375
• Publication Number:
  US 20100055747A1
• Time in Patent Office: Days
• US Class Current: 435/105
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/12 Disaccharides
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2201/00 Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The present teachings provide methods of converting cellulosic materials to soluble sugars. Methods for increasing the yield of glucose from the enzymatic saccharification of cellulosic materials is also provided. The present teachings further provide methods of increasing the yield of cellobiose from the enzymatic saccharification of cellulosic materials.

Claims

1. A method for converting a cellulosic material to glucose comprising the steps of:

combining a cellulosic material with a cellulase such that the resulting combination of cellulosic material and cellulase has 1% to about 30% cellulose by weight; and

incubating said cellulosic material and cellulase combination at a temperature greater than about 38°

C. to about 100°

C. for about 0.1 hours to about 96 hours at a pH of from about 4 to about 9 to cause a hydrolysis reaction to convert at least 20% of said cellulosic material to soluble sugars,

wherein said soluble sugars comprises glucose and cellobiose, and the fraction of glucose is at least 0.75 relative to said soluble sugars.

2. The method of claim 1 wherein the cellulosic material selected from the group consisting of bioenergy crops, agricultural residues, municipal solid waste, industrial solid waste, yard waste, wood, forestry waste, switchgrass, waste paper, sludge from paper manufacture, corn grain, corn cobs, corn husks, corn stover, grasses, wheat, wheat straw, hay, rice straw, sugar cane bagasse, sorghum, soy, trees, switchgrass, hay, barley, barley straw, rice straw, and grasses.

3. The method of claim 1 further comprising pretreating said cellulosic material.

4. The method of claim 3 wherein said pretreatment is selected from a group consisting of steam explosion, pulping, grinding, acid hydrolysis, and combinations thereof.

5. The method of claim 1 further comprising determining the amount of glucose.

6. The method of claim 1 further comprising determining the amount of soluble sugars.

7. The method of claim 1 wherein the amount of cellulase is about 2-40 mg/g cellulosic material.

8. The method of claim 1 wherein said cellulase comprises a whole cellulase.

9. The method of claim 8 wherein said whole cellulase is a Trichoderma reesei whole cellulase.

10. The method of claim 9 wherein said Trichoderma reesei expresses a recombinant enzyme.

11. The method of claim 10 wherein said recombinant enzyme is a beta-glucosidase.

12. A method for converting a cellulosic material to cellobiose comprising the steps of:

combining a cellulosic material with a cellulase mixture comprising an endoglucanase 1 such that the resulting combination of cellulosic material and cellulase mixture has 1% to about 30% cellulose by weight; and

incubating said cellulosic material and cellulase combination at a temperature less than about 100°

C. to about 25°

C. for about 0.1 hours to about 96 hours at a pH of from about 4 to about 9 to cause a hydrolysis reaction to convert up to 50% of said cellulosic material to soluble sugars, wherein said soluble sugars comprises glucose and cellobiose and the fraction of glucose is less than about 0.5 relative to said soluble sugars.

13. The method of claim 12 wherein the cellulosic material selected from the group consisting of bioenergy crops, agricultural residues, municipal solid waste, industrial solid waste, yard waste, wood, forestry waste, switchgrass, waste paper, sludge from paper manufacture, corn grain, corn cobs, corn husks, corn stover, grasses, wheat, wheat straw, hay, rice straw, sugar cane bagasse, sorghum, soy, trees, switchgrass, hay, barley, barley straw, rice straw, and grasses.

14. The method of claim 12 further comprising pretreating said cellulosic material.

15. The method of claim 14 wherein said pretreatment is selected from a group consisting of steam explosion, pulping, grinding, acid hydrolysis, and combinations thereof.

16. The method of claim 12 further comprising determining the amount of glucose.

17. The method of claim 12 further comprising determining the amount of soluble sugars.

18. The method of claim 12 wherein the amount of said cellulase mixture is about 2 mg-40 mg/g cellulosic material.

19. The method of claim 13 wherein said cellulase mixture further comprises a cellobiohydrolase 1.

20. The method of claim 13 wherein said cellulase mixture further comprises a cellobiohydrolase 2.

21. The method of claim 13 wherein said cellulase mixture further comprises Thermomonospera fusca E3.