NUCLEIC ACID ARRAY HAVING FIXED NUCLEIC ACID ANTI-PROBES AND COMPLEMENTARY FREE NUCLEIC ACID PROBES
First Claim
1. A process for identifying a complementary nucleic acid probe to a target nucleic acid comprising:
- forming an array of spots, each spot comprising a nucleic acid probe, a nucleic acid probe hybridized to a respective immobilized oligonucleotide anti-probe to yield a double-stranded anti-probe-nucleic acid probe complex;
placing said array in a solution filled array chamber;
denaturing said double-stranded oligonucleotide anti-probe-nucleic acid probe complex;
moving said nucleic acid probe electrophoretically into a target chamber comprising a target nucleic acid;
establishing hybridization conditions in said target chamber to form a target nucleic acid-nucleic acid probe double-stranded complex when the target nucleic acid has a complementary sequence to said nucleic acid probe;
transporting a nucleic acid probe noncomplementary to the target nucleic acid into contact with a series of immobilized anti-probes;
hybridizing each of said nucleic acid probes noncomplementary to the target nucleic acid to one of said series of immobilized anti-probes; and
determining whether each of said series of immobilized anti-probes exist as present as a single strand.
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Accused Products
Abstract
A process for identifying a complementary nucleic acid probe to a target nucleic acid involves forming an array of spots where each spot of the array has an immobilized nucleic acid anti-probe to which is hybridized a nucleic acid probe. The array of the anti-probe-probe complex is denatured. The nucleic acid probes are then moved into a target chamber that includes a target nucleic acid. Hybridization conditions are established to form double-stranded complexation between the target nucleic acid and nucleic acid probes in instances where the target nucleic acid has a sequence complementary. The nucleic acid probes noncomplementary to the target nucleic acid are allowed to rehybridize with anti-probes. Determining whether the anti-probe spots exposed to nucleic acid probes noncomplementary to the target nucleic acid are single stranded after exposure to noncomplementary nucleic acid probes provides information as to target nucleic acid sequence.
42 Citations
22 Claims
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1. A process for identifying a complementary nucleic acid probe to a target nucleic acid comprising:
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forming an array of spots, each spot comprising a nucleic acid probe, a nucleic acid probe hybridized to a respective immobilized oligonucleotide anti-probe to yield a double-stranded anti-probe-nucleic acid probe complex; placing said array in a solution filled array chamber; denaturing said double-stranded oligonucleotide anti-probe-nucleic acid probe complex; moving said nucleic acid probe electrophoretically into a target chamber comprising a target nucleic acid; establishing hybridization conditions in said target chamber to form a target nucleic acid-nucleic acid probe double-stranded complex when the target nucleic acid has a complementary sequence to said nucleic acid probe; transporting a nucleic acid probe noncomplementary to the target nucleic acid into contact with a series of immobilized anti-probes; hybridizing each of said nucleic acid probes noncomplementary to the target nucleic acid to one of said series of immobilized anti-probes; and determining whether each of said series of immobilized anti-probes exist as present as a single strand. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. A nucleic acid assay assemblage comprising:
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an array chamber containing nucleic acid probes each immobilized to a complementary nucleic acid anti-probe in the form of a double-stranded complex; a target chamber containing a target nucleic acid; a channel permeable to said nucleic acid probes in fluid communication between said array chamber and said target chamber; and a fixture for coupling an electrophoretic electrode to said assay chamber and a second electrophoretic electrode to said target chamber. - View Dependent Claims (18, 19, 20, 21, 22)
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Specification