N-TERMINAL DERIVATISATION OF PROTEINS WITH POLYSACCHARIDES
First Claim
1. A method for producing a purified N-terminal derivative of a protein or peptide in which(i) a polysaccharide is reacted at the amine group of the N-terminus of a protein or peptide in aqueous acidic solution to produce an N-terminal derivative;
- and(ii) the resultant N-terminal derivative is purified in aqueous solution of higher pH than in step (i).
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to methods for producing N-terminal derivatives of proteins in which a polysaccharide, preferably having at least terminal sialic units and preferably consisting essentially only of sialic acid units, is reacted at the N-terminus of a protein or peptide under controlled conditions to produce an N-terminal derivative. The controlled conditions include use of acidic pH for the derivatisation step and a higher pH for purification. The derivatives are useful for improving pharmacokinetics and pharmacodynamics of proteins and peptides.
-
Citations
29 Claims
-
1. A method for producing a purified N-terminal derivative of a protein or peptide in which
(i) a polysaccharide is reacted at the amine group of the N-terminus of a protein or peptide in aqueous acidic solution to produce an N-terminal derivative; - and
(ii) the resultant N-terminal derivative is purified in aqueous solution of higher pH than in step (i). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 29)
- and
- 20. A composition comprising a population of polysialic acid derivatives of a protein, wherein the derivatives comprise between 2 and 200 sialic acid units and wherein the population consists substantially only of N-terminal derivatives of the protein.
Specification