CLARIFICATION OF TRANSGENIC MILK USING DEPTH FILTRATION
First Claim
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1. A method for separating a protein of interest from a feedstream, comprising:
- (a) filtering said feedstream by a depth filtration process that separates said protein of interest from said feedstream on the basis of size;
(b) performing a viral inactivation step that inactivates at least 90% of viral particles or fragments;
(c) performing an aseptic filtration step for the filtered feedstream; and
,(d) clarifying the remaining material through additional downstream processingwherein material that is retained by a filter during the depth filtration process is trapped both upon a surface of the filter and within a matrix of the filter.
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Abstract
Processes and apparati are provided for separating molecules of interest from a mixture by depth filtration (DF). The DF of the invention is useful in the clarification and processing of various feedstreams for the removal of a molecule of interest. According to a preferred embodiment, a transgenic milk feedstream is stabilized and particulate matter such as fat, casein miscelles and bacteria are removed. An aseptic filtration step was also developed to remove any bacteria remaining in a clarified transgenic milk feedstream.
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Citations
82 Claims
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1. A method for separating a protein of interest from a feedstream, comprising:
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(a) filtering said feedstream by a depth filtration process that separates said protein of interest from said feedstream on the basis of size; (b) performing a viral inactivation step that inactivates at least 90% of viral particles or fragments; (c) performing an aseptic filtration step for the filtered feedstream; and
,(d) clarifying the remaining material through additional downstream processing wherein material that is retained by a filter during the depth filtration process is trapped both upon a surface of the filter and within a matrix of the filter. - View Dependent Claims (5, 6, 7, 12, 21, 28, 29, 30, 33, 34)
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39. A method for separating a protein of interest from a transgenic milk feedstream, comprising:
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(a) filtering said milk feedstream by a depth filtration process that separates said protein of interest from said feedstream on the basis of size; (b) performing a viral inactivation step that inactivates at least 90% of viral particles or fragments; (c) performing an aseptic filtration step for the filtered feedstream; and
,(d) clarifying the remaining material through downstream processing; wherein material that is retained by a filter during the depth filtration process is trapped both upon a surface of the filter and within a matrix of the filter. - View Dependent Claims (43, 44, 45, 50, 59)
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74. A method for isolating a protein of interest from a feedstream comprising:
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collecting a feedstream sample; diluting the feedstream sample with ammonium sulfate resulting in the precipitation of contaminants, wherein the pH of the collected feedstream is above pH 5.0 subjecting the collected feedstream to microfiltration in a filtration unit with a mean pore size ranging from 1.5 micron to 15 micron; subjecting the filtrate to tangential flow microfiltration with a limit of separation of 5,000 to 50,000 daltons. - View Dependent Claims (75, 76)
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Specification