Biomaterial Measuring Device and Manufacturing Method Thereof

• US 20100062520A1
• Filed: 12/10/2004
• Published: 03/11/2010
• Est. Priority Date: 12/11/2003
• Status: Active Grant

• Current Assignee: Ceragem Medisys Inc.
• Original Assignee: Ceragem Medisys Inc.
• Inventors: Lee, Jin-Woo
• Granted Patent: US 8,172,994 B2
• Time in Patent Office: Days
• US Class Current: 435/287.100
• CPC Class Codes: G01N 2021/7783 Transmission, loss
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10T 29/49208 by assembling plural parts

Abstract

A method of producing a biomaterial measuring device is disclosed. The method includes forming a plurality of reactions elements, to which an assay reagent is applied, on a first substrate, cutting the resulting first substrate in a unit of individual reaction element, and attaching a first substrate piece, which is formed by cutting the resulting first substrate in the unit of individual reaction element, to a predetermined portion of a second substrate. In the biomaterial measuring device, since a material cost in minimized and it is easy to automate production, it is possible to reduce a production, it is possible to reduce a production cost.

Claims

1. A method of producing a biomaterial measuring device, comprising:

forming a plurality of reaction elements, to which an assay reagent is applied, on a first substrate;

cutting the resulting first substrate in a unit of individual reaction element; and

attaching a first substrate piece, which is formed by cutting the resulting first substrate in the unit of individual reaction element, to a predetermined portion of a second substrate.

2. The method as set forth in claim 1, wherein a plurality of first substrate pieces, which are formed by cutting the resulting first substrate in the unit of individual reaction element, is attached to the second substrate.

3. The method as set forth in claim 1, wherein the assay reagent is used to measure a biomaterial through an optical assay, and light penetrates through the portion of the second substrate, to which the first substrate piece is attached.

4. The method as set forth in claim 3, wherein the assay reagent is layered on a membrane so as to be applied to a first substrate, or is directly applied to the first substrate.

5. The method as set forth in claim 3, further comprising forming means for focusing or defocusing light, on the portion of the second substrate, to which the first substrate piece is attached.

6. The method as set forth in claim 1, wherein the second substrate is made of any one selected from the group consisting of plastic, glass, and semiconductor wafer.

7. The method as set forth in claim 1, wherein the assay reagent is used to measure a biomaterial through an electrochemical assay, and the formation of the plurality of reaction elements comprises:

forming at least two first electrodes on a first side of the first substrate; and

applying the assay reagent through the first electrodes.

8. The method as set forth in claim 7, further comprising:

forming a second electrode on a second side of the first substrate, which is opposite to the first side; and

electrically connecting at least one of the first electrodes on the first side to the second electrode on the second side.

9. The method as set forth in claim 1, further comprising mounting a dehumidifying agent for removing moisture, which is introduced from an environment to the assay reagent, on a predetermined portion of the biomaterial measuring device while the dehumidifying agent is isolated from the environment.

10. A method of producing a biomaterial measuring device, comprising:

forming a plurality of first reaction elements, to which a first assay reagent is applied, on a first substrate;

forming a plurality of second reaction elements, to which a second assay reagent is applied, on a second substrate;

cutting the first and second substrates in a unit of individual reaction element; and

attaching first and second substrate pieces, which are formed by cutting the first and second substrates in the unit of individual reaction element, to predetermined portions of a third substrate.

11. A biomaterial measuring device, comprising:

a first substrate;

an assay reagent which is applied on an entire first side of the first substrate to form a reaction element; and

a second substrate, on which the first substrate is mounted to enable the assay reagent to form a path for introducing a biomaterial therethrough.

12. The biomaterial measuring device as set forth in claim 11, wherein the assay reagent is used to measure the biomaterial through an optical assay, and light penetrates through a portion of the second substrate, to which the first substrate is attached.

13. The biomaterial measuring device as set forth in claim 12, wherein the assay reagent is layered on a membrane so as to be applied to the first substrate, or is directly applied to the first substrate.

14. The biomaterial measuring device as set forth in claim 12, further comprising means for focusing or defocusing light, which is formed on the portion of the second substrate, to which the first substrate is attached.

15. The biomaterial measuring device as set forth in claim 11, wherein the second substrate is made of any one selected from the group consisting of plastic, glass, and semiconductor wafer.

16. The biomaterial measuring device as set forth in claim 11, wherein the reaction element includes at least two first electrodes formed on the first side of the first substrate, and the assay reagent is used to measure the biomaterial through an electrochemical assay and is applied through the first electrodes.

17. The biomaterial measuring device as set forth in claim 16, further comprising a second electrode which is formed on a second side of the first substrate, opposite to the first side, and which is electrically connected to at least one of the first electrodes on the first side.

18. The biomaterial measuring device as set forth in claim 17, wherein the first electrodes on the first side are electrically connected to the second electrode on the second side through a via hole which is formed through the first substrate, a wall of which is coated with a conductor.

19. The biomaterial measuring device as set forth in claim 11, further comprising a dehumidifying agent which is mounted on a predetermined portion of the biomaterial measuring device so that the dehumidifying agent is isolated from an environment and which removes moisture introduced from the environment to the assay reagent.