NUCLEOTIDE SEQUENCES AND CORRESPONDING POLYPEPTIDES CONFERRING IMPROVED AGRICULTURAL AND/OR ORNAMENTAL CHARACTERISTICS TO PLANTS BY MODULATING ABSCISSION

• US 20100064392A1
• Filed: 06/09/2009
• Published: 03/11/2010
• Est. Priority Date: 06/10/2008
• Status: Abandoned Application

• Current Assignee: Ceres (Land O'Lakes Incorporated)
• Original Assignee: Ceres (Land O'Lakes Incorporated)
• Inventors: YANG, Dennis, Wang, Wuyi
• Application Number:
  US12/481,338
• Publication Number:
  US 20100064392A1
• Time in Patent Office: Days
• US Class Current: 800/290
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C12N 15/8266 Abscission; Dehiscence; Senescence

Abstract

Methods and materials for modulating abscission and/or inflorescence development time levels in plants are disclosed. For example, nucleic acids encoding abscission and/or inflorescence development time-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having modulated abscission and/or inflorescence development time levels and plant products produced from plants having such levels.

Claims

1. A plant cell comprising an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide has an HMM bit score greater than about 115 based on the amino acid sequences depicted in FIG. 1 or FIG. 2.

4. A transgenic plant comprising the plant cell of claim 1.

5. The transgenic plant of claim 4, wherein said plant is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor (sorghum, sudangrass), Miscanthus giganteus (miscanthus), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).

6. A transgenic plant comprising the plant cell of claim 4, wherein said polypeptide is selected from the group consisting of SEQ ID NO:

3 and 31.

7. A seed product comprising embryonic tissue from a transgenic plant according to claim 4, wherein said seed product contains said exogenous nucleic acid.

2. A plant cell comprising an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence encoding a polypeptide having 80 percent or greater sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO:

3, 4, 6, 8, 10, 11, 12, 14, 16, 18, 19, 21, 22, 24, 25, 26, 27, 28, 31, 32, 34, 36, 38, 40, 42, 44, 46, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, and 69.

10. A transgenic plant comprising the plant cell of claim 2.

11. The transgenic plant of claim 10, wherein said plant is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor (sorghum, sudangrass), Miscanthus giganteus (miscanthus), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).

12. A transgenic plant comprising the plant cell of claim 10, wherein said polypeptide is selected from the group consisting of SEQ ID NO:

3 and 31.

13. A seed product comprising embryonic tissue from a transgenic plant according to claim 10, wherein said seed product contains said exogenous nucleic acid.

3. A plant cell comprising an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence having 80 percent or greater sequence identity to a nucleotide sequence selected from the group consisting of SEQ ID NO:

1, 2, 5, 7, 9, 13, 15, 17, 20, 23, 29, 30, 33, 35, 37, 39, 41, 43, 45, 48, 50, 52, 54, 56, 58, 60, 62, 64, 66, and 68, or a fragment thereof.

14. A transgenic plant comprising the plant cell of claim 3.

15. The transgenic plant of claim 14, wherein said plant is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor (sorghum, sudangrass), Miscanthus giganteus (miscanthus), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).

16. A transgenic plant comprising the plant cell of claim 14, wherein said polypeptide is selected from the group consisting of SEQ ID NO:

3 and 31.

17. A seed product comprising embryonic tissue from a transgenic plant according to claim 14, wherein said seed product contains said exogenous nucleic acid.

8. An isolated nucleic acid comprising a nucleotide sequence having 85% or greater sequence identity to the nucleotide sequence set forth in SEQ ID NO:

35, 48, 7, 39, 13, 52, 62, 43, 17, 33, 5, 20, or 23.

18. A method of modulating the level of abscission in a plant comprising introducing into a plant cell an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence according to claim 8.

9. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide having 80% or greater sequence identity to the amino acid sequence set forth in SEQ ID NO:

36, 49, 8, 40, 14, 53, 63, 44, 18, 34, 6, 21, or 24.

19. A method of modulating the level of abscission in a plant comprising introducing into a plant cell an exogenous nucleic acid, wherein said exogenous nucleic acid comprises a regulatory region operably linked to a nucleotide sequence according to claim 9.

20. The method according to claim 19, wherein said plant cell is from a plant that is a member of a species selected from the group consisting of Panicum virgatum (switchgrass), Sorghum bicolor (sorghum, sudangrass), Miscanthus giganteus (miscanthus), Saccharum sp. (energycane), Populus balsamifera (poplar), Zea mays (corn), Glycine max (soybean), Brassica napus (canola), Triticum aestivum (wheat), Gossypium hirsutum (cotton), Oryza sativa (rice), Helianthus annuus (sunflower), Medicago sativa (alfalfa), Beta vulgaris (sugarbeet), and Pennisetum glaucum (pearl millet).