CATIONIC POLYMER BASED WIRED ENZYME FORMULATIONS FOR USE IN ANALYTE SENSORS

• US 20100065441A1
• Filed: 09/15/2008
• Published: 03/18/2010
• Est. Priority Date: 09/15/2008
• Status: Active Grant

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Feldman, Benjamin J., Liu, Zenghe, Ouyang, Tianmei
• Granted Patent: US 8,636,884 B2
• Time in Patent Office: Days
• US Class Current: 205/777.500
• CPC Class Codes: A61K 38/28 Insulins
• CPC Class Codes: C12Q 1/004 mediator-assisted
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: C12Q 1/32 involving dehydrogenase
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 27/3273 Devices therefor, e.g. test element readers, circuitry details not specific to biochemical electrodes G01N33/4875

Abstract

Embodiments of the invention include analyte-responsive compositions and electrochemical analyte sensors having a sensing layer that includes an analyte-responsive enzyme and a cationic polymer. Also provided are systems and methods of making the sensors and using the electrochemical analyte sensors in analyte monitoring.

Claims

1-45. -45. (canceled)

46. A glucose-responsive composition comprising:

a glucose-responsive enzyme, a cationic polymer and a redox mediator to accept electrons from the enzyme.

47. The composition of claim 46, wherein the glucose-responsive enzyme is glucose oxidase (GOx).

48. The composition of claim 46, wherein the glucose-responsive enzyme is glucose dehydrogenase (GDH).

49. The composition of claim 48, wherein the glucose dehydrogenase is associated with a co-factor.

50. The composition of claim 49, wherein the co-factor is flavin adenine dinucleotide (FAD), nicotinamide adenine dinucleotide (NAD), or pyrroloquinoline quinone (PQQ).

51. The composition of claim 46, wherein the glucose-responsive enzyme comprises a complex of glucose dehydrogenase (GDH) and flavin adenine dinucleotide (FAD).

52. The composition of claim 46, wherein the redox mediator comprises ferricyanide, phenanthroline quinone, or ferrocene.

53. The composition of claim 46, wherein the redox mediator comprises a transition metal complex.

54. The composition of claim 53, wherein the transition metal complex comprises osmium.

55. The composition of claim 53, wherein the transition metal complex comprises the following formula:

wherein

(i) M is ruthenium, osmium, or vanadium; and

(ii) L is selected from the group consisting of;

wherein;

R₁, R₂, and R′

₁ are independently substituted or unsubstituted alkyl, alkenyl, or aryl groups;

R₃, R₄, R₅, R₆, R′

₃, R′

₄, R_a, R_b, R_c, and R_d are independently —

H, —

F, —

Cl, —

Br, —

I, —

NO₂, —

CN, —

CO₂H, —

SO₃H, —

NHNH₂, —

SH, aryl, alkoxycarbonyl, alkylaminocarbonyl, dialkylaminocarbonyl, —

OH, alkoxy, —

NH₂, alkylamino, dialkylamino, alkanoylamino, arylcarboxamido, hydrazino, alkylhydrazino, hydroxylamino, alkoxyamino, alkylthio, alkenyl, aryl, or alkyl;

c is an integer selected from −

1 to −

5 or +1 to +5 indicating a positive or negative charge;

X represents at least one counter ion;

d is an integer from 1 to 5 representing the number of counter ions; and

L₁, L₂, L₃ and L₄ are ligands, wherein L₁ comprises a heterocyclic compound coupled a polymeric backbone; and

wherein L₁ and L₂ in combination form a first bidentate ligand.

56. The composition of claim 55, wherein the transition metal complex comprises the following formula:

wherein R₃, R₄, R₅, R₆, R_a, R_b, R_c, R_d, R′

₃ and R′

₄ are —

H;

R₁ and R₂ are independently substituted or unsubstituted C1 to C12 alkyls; and

R₁, R₂, and R′

₁ are independently —

H or substituted or unsubstituted C1-C12 alkoxy, C1-12 alkylthio, C1-C12 alkylamino, C2-C24 dialkylamino, or C1-C12 alkyl.

57. The composition of claim 56, wherein at least one of R₁, R₂, and R′

₁ comprises a reactive group selected from the group consisting of carboxy, activated ester, sulfonyl halide, sulfonate ester, isocyanate, isothiocyanate, epoxide, aziridine, halide, aldehyde, ketone, amine, acrylamide, thiol, acyl azide, acyl halide, hydrazine, hydroxyamine, alkyl halide, imidazole, pyridine, phenol, alkyl sulfonate, halotriazine, imido ester, maleimide, hydrazide, hydroxy, and photo-reactive azido aryl groups.

58. The composition of claim 46, wherein said cationic polymer is polyallylamine (PAH), polyethyleneimine (PEI), poly(L-lysine) (PLL), or poly(L-arginine) (PLA).

59. The composition of claim 46, wherein the redox mediator is covalently associated with the cationic polymer.

60. The composition of claim 46, wherein the redox mediator is non-covalently associated with the cationic polymer.