COMPOSITE MATERIAL

• US 20100065499A1
• Filed: 03/07/2008
• Published: 03/18/2010
• Est. Priority Date: 03/07/2007
• Status: Abandoned Application

• Current Assignee: Jacobs University Bremen Ggmbh
• Original Assignee: Jacobs University Bremen Ggmbh
• Inventors: Grasselli, Mariano, Fernandez-Lahore, Marcello
• Application Number:
  US12/449,956
• Publication Number:
  US 20100065499A1
• Time in Patent Office: Days
• US Class Current: 210/656
• CPC Class Codes: B01D 15/20 relating to the conditioning of the sorbent material
• CPC Class Codes: B01J 20/264 derived from different types of monomers, e.g. linear or branched copolymers, block copolymers, graft copolymers
• CPC Class Codes: B01J 20/28004 Sorbent size or size distribution, e.g. particle size
• CPC Class Codes: B01J 20/28023 Fibres or filaments fibres or filaments in the form of membranes B01J20/28038; B01J20/28007 takes precedence
• CPC Class Codes: B01J 20/28033 Membrane, sheet, cloth, pad, lamellar or mat
• CPC Class Codes: B01J 20/28052 Several layers of identical or different sorbents stacked in a housing, e.g. in a column
• CPC Class Codes: B01J 20/285 based on polymers
• CPC Class Codes: D06M 14/22 of vegetal origin, e.g. cellulose or derivatives thereof
• CPC Class Codes: Y02C 20/40 of CO2
• CPC Class Codes: Y10T 428/298 Physical dimension

Abstract

The invention describes a composite microfibre, which is comprising a polysaccharide polymer selected from the group consisting of cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, n-propyl cellulose, isopropyl cellulose, cellulose acetate and combinations of two or more of these substances, and combinations of two or more of these substances, thereby the polysaccharide polymer further comprising substituents comprising a given formula, wherein k is 1 or greater, and wherein each moiety R₁ is independently selected from H and methyl, and wherein each moiety R₂ is independently selected from —H, —OH, —NH₂, —SH, —OR₃, —NHR₃ and —NR₃R₄, —SR₃, and wherein each moiety R₃ and R₄ are chemical groups independently selected from C1-C4 alkyl, C1-C4 alkoxy, C1-C4 alkoxy halide and C1-C4 heteroalkyl substituents, and is preferably selected from —CH₃, —CH₂—CH₃, —CH₂—OH, —CH₂—CH₂—OH, —CH₂—CH₂—NH₂, —CH—CH(OH)—CH₂—OH, —CH—CH(OH)—CH₃, glycidyl, anhydride, sulfonic acid and hydroxysuccinimide, said substituents forming a hydrogel in the presence of water, and said composite microfibre having a swelling factor of at least 10%, preferably of 10-400%.

Claims

2. Composite microfibre,

comprising a polysaccharide polymer selected from the group consisting of cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, n-propyl cellulose, isopropyl cellulose, cellulose acetate and combinations of two or more of these substances, and combinations of two or more of these substances,

the polysaccharide polymer further comprising substituents of formula (I)

3. Method of producing a composite microfibre, comprising the steps of

(i) providing a hydrophilic polysaccharide polymer fibre, the polysaccharide being selected from the group consisting of cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, ethyl cellulose, n-propyl cellulose, isopropyl cellulose, cellulose acetate and combinations of two or more of these substances, and combinations of two or more of these substances,

(ii) gamma radiation induced polymerization of two or more monomers for simultaneous producing and grafting of substituents of formula (I),

1. Composite microfibre, obtainable or obtained by a method according to claim 3.

4. Method according to claim 3, wherein

a) the monomers for gamma radiation induced polymerization are vinyl monomers, preferably glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) and/or

b) the liquid medium is a polar liquid medium and preferably is, is substantially or comprises water and optionally one or more of methanol, ethanol, n-propanol, iso-propanol, butanol, ter-butanol, acetonitrile, dimethylsulfoxide, acetone, dimethylformamide, and mixtures thereof.

5. Method according to claim 3, wherein the gamma radiation induced polymerization is performed in the absence of a polymerization initiator substance, preferably particularly in the absence of peroxides and/or azo compounds.

6. Method according to claim 3, wherein the gamma radiation induced polymerization is performed with

a) a radiation dose of 5-15 kGy, preferably 8-12 kGy, and/or

b) a dose velocity of 0.1-1 kGy/h.

7. Method according to claim 3, wherein the hydrophilic polysaccharide fibre of step (i) is pre-treated by alkali hydrophilization, preferably by pre-treating with sodium hydroxide, ammonium hydroxide or potassium hydroxide.

8. Method according to claim 3, wherein step (ii) is performed in such way that the total mass of substituents of formula (I) is 5-200 wt % based on the total mass of hydrophilic polysaccharide fibre provided in step (i).

9. Composite microfibre according to claim 1, wherein the total mass of substituents of formula (I) is 5-200 wt % based on the total mass of hydrophilic polysaccharide.

10. Composite microfibre according to claim 1, wherein each substituent of formula (I) is selected from the group consisting of (poly)acrylamides, (poly)methacrylamides, (poly)acrylates, (poly)methacrylates, and polymers producible with one or more monomers selected from the group consisting of 2-hydroxyethyl (meth)acrylate, hydroxyethoxyethyl (meth)acrylate, hydroxydiethoxyethyl (meth)acrylate, methoxyethyl (meth)acrylate, methoxyethoxyethyl (meth)acrylate, methoxydiethoxyethyl (meth)acrylate, polyethyleneglycol(meth)acrylate, methoxy-polyethylene glycol(meth)acrylate, (meth)acrylic acid, sodium (meth)acrylate, glycerol (meth)acrylate, hydroxypropyl (meth)acrylate, and hydroxybutyl (meth)acrylates, 2-acrylamido-2-methyl-1-propanesulfonic acid, 2-(acryloyloxy)ethyltrimethyl-ammonium methyl sulfate, (2-trifluoromethyl)acrylic acid, 3-sulfopropyl acrylate potassium salt, glycidyl (meth)acrylate, morpholine (meth)acrylate (meth)acrylonitrile, anhydride (meth)acrylic, 2-(methacryloxy)ethyl phosphate, N,N′

-diethylaminoethyl (meth)acrylate, (3-(methacryloylamino)propyl)trimethylammonium chloride, (3-acrylamidopropyl)trimethylammonium chloride and acrylamide monomers such as acrylamide and substituted acrylamines, such as dimethyl (meth)acrylamide, diethyl (meth)acrylamide, diacetone (meth)acrylamide, aminopropyl (meth)acrylamide, isopropyl (meth)acrylamide, or copolymers of these substituents.

11. Composite microfibre according to claim 1, wherein the substituent of formula (I) is crosslinked with a crosslinker monomer selected from the group consisting of divinylbenzene, ethyleneglycol di(meth)acrylate, 1,4-butanediol di(meth)acrylate, triethyleneglycol di(meth)acrylate, diethyleneglycol di(meth)acrylate, triethyleneglycol di(meth)acrylate and poly(ethylene glycol) di(meth)acrylate, oligo(polyhydroxyalkyl)silyl (meth)acrylate, and ethylene-bis-(meth)acrylamide, methylene-bis-(meth)acrylamide, and piperazine di(meth)acrylamide.

12. Composite microfibre according to claim 1, wherein a substituent of formula (I) comprises a glycidyl, oxime, halogen or anhydride functionality.

13. Composite microfibre according to claim 1, having

(a) a thickness of 5-80 μ

m in its swollen state and/or

(b) a length of 0.5-10 cm.

14. Composite microfibre according to claim 1, further comprising immobilized thereon one or more modifiers selected from the group consisting of antibodies or F(ab′

)2, Fab, Fv or scFv antibody fragments, (recombinant) protein A, (recombinant) protein G, dyes, chelating groups and imidoacetic acid complexes with Cu2+, Ni2+, Zn2+ or Co2+.

15. Chromatographic material, comprising, consisting essentially or consisting of a composite microfibre according to claim 1.

16. Chromatographic material according to claim 15, further comprising a swelling ratio of 10 to 400% and preferentially of 50 to 200% in distilled water at 20°

C.

17. Chromatographic material according to claim 15, wherein the composite microfibres are arranged in the form of stacked disks, rolls, or in parallel or twirled arrangement.

18. Chromatography device, comprising a chromatographic adsorption section, said section comprising the chromatographic material according to claim 15.

19. Use of a composite microfibre according to claim 1, as adsorptive material in chromatography.