MICROFLUIDIC DEVICE WITH A CYLINDRICAL MICROCHANNEL AND A METHOD FOR FABRICATING SAME

• US 20100068740A1
• Filed: 11/05/2007
• Published: 03/18/2010
• Est. Priority Date: 11/03/2006
• Status: Abandoned Application

• Current Assignee: Trustees of Tufts College, Tufts University
• Original Assignee: Trustees of Tufts College
• Inventors: Perry, Hannah, Kaplan, David L., Georgakoudi, Irene, Omenetto, Fiorenzo, Cronin-Golomb, Mark, Lawrence, Brian
• Application Number:
  US12/513,416
• Publication Number:
  US 20100068740A1
• Time in Patent Office: Days
• US Class Current: 435/14
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 2300/163 Biocompatibility
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B29C 39/02 for making articles of definite length, i.e. discrete articles
• CPC Class Codes: B29D 11/0074 Production of other optical elements not provided for in B29D11/00009- B29D11/0073
• CPC Class Codes: B81B 2201/058 Microfluidics not provided for in B81B2201/051 - B81B2201/054
• CPC Class Codes: B81B 2201/06 Bio-MEMS
• CPC Class Codes: B81C 1/00071 Channels
• CPC Class Codes: C12M 23/16 Microfluidic devices; Capillary tubes integrated microfluidic structures B01L3/5027; microreactors B01J19/0093
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0237 Adjustable, e.g. focussing
• CPC Class Codes: G01J 3/0256 Compact construction
• CPC Class Codes: G01J 3/06 Scanning arrangements arrangements for order-selection
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/1804 Plane gratings
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

A method of manufacturing a microfluidic device having at least one cylindrical microchannel includes providing a substrate, casting an uncured polymer matrix solution onto the substrate, embedding an elongated rod in the uncured polymer matrix solution, curing the polymer matrix solution to form a solidified body, and extracting the elongated rod to form the cylindrical microchannel in the solidified body. In another embodiment, the method includes forming an optical feature on a surface of the microfluidic device. A microfluidic device is also provided, the device including a polymer body, and at least one cylindrical microchannel in the polymer body, the cylindrical microchannel having a diameter between approximately 40 ?m and 250 ?m, inclusive. An additional microfluidic device is provided that functions as an optofluidic spectrometer. The optofluidic spectrometer includes a polymer body, a diffraction grating integrated within the polymer body, and a cylindrical microchannel behind the diffraction grating on the polymer body.

Claims

1. A method of manufacturing a microfluidic device having at least one cylindrical microchannel comprising:

providing a substrate;

casting an uncured polymer matrix solution onto said substrate;

embedding an elongated rod in said uncured polymer matrix solution;

curing said polymer matrix solution to form a solidified body of said microfluidic device; and

extracting said elongated rod to form said cylindrical microchannel in said solidified body.

2. The method of claim 1, wherein said elongated rod is a silica rod.

3. The method of claim 2, wherein said silica rod has a diameter between approximately 40 μ

m and 250 μ

m, inclusive.

4. The method of claim 3, wherein said silica rod has a diameter between approximately 57 μ

m and 125 μ

m, inclusive.

5. The method of claim 1, wherein said polymer matrix solution includes polydimethylsiloxane (PDMS).

6. The method of claim 1, wherein said polymer matrix solution includes a biopolymer.

7. The method of claim 6, wherein said biopolymer is selected from a group consisting of chitosan, collagen, gelatin, agarose, chitin, polyhydroxyalkanoates, pullan, starch (amylose amylopectin), cellulose, hyaluronic acid, and related biopolymers, or a combination thereof.

8. The method of claim 6, wherein said biopolymer is silk.

9. The method of claim 1, wherein said polymer matrix solution is an aqueous silk fibroin solution having approximately 1.0 wt % to 30 wt % silk, inclusive.

10. The method of claim 9, wherein said aqueous silk fibroin solution has approximately 8.0 wt %.

11. The method of claim 1, wherein said curing said polymer matrix solution includes applying heat to said uncured polymer matrix solution.

12. The method of claim 1, further comprising:

coating said silica rod with a surfactant solution.

13. The method of claim 1, further comprising

forming an optical element on a surface of said microfluidic device.

14. The method of claim 13, wherein said substrate is a template for said optical element.

15. The method of claim 14, wherein said optical element is at least one of a lens, a microlens array, an optical grating, a pattern generator, a beam reshaper, a mirror blank, and a glass slide.

16. The method of claim 1, further comprising:

adding a doping agent to said uncured polymer matrix solution.

17. The method of claim 16, wherein said doping agent is selected from a group consisting of red blood cells, horseradish peroxidase, and phenolsulfonphthalein, or a combination thereof.

18. The method of claim 16, wherein said doping agent is selected from a group consisting of a nucleic acid, a dye, a cell, an antibody, enzymes, for example, peroxidase, lipase, amylose, organophosphate dehydrogenase, ligases, restriction endonucleases, ribonucleases, DNA polymerases, glucose oxidase, laccase, cells, viruses, proteins, peptides, small molecules, drugs, dyes, amino acids, vitamins, antixoxidants, DNA, RNA, RNAi, lipids, nucleotides, aptamers, carbohydrates, chromophores, light emitting organic compounds such as luciferin, carotenes and light emitting inorganic compounds, chemical dyes, antibiotics, antifungals, antivirals, light harvesting compounds such as chlorophyll, bacteriorhodopsin, protorhodopsin, and porphyrins and related electronically active compounds, or a combination thereof.

19. The method of claim 1, further comprising:

suspending said elongated rod over said substrate.

20. A microfluidic device comprising:

a polymer body; and

at least one cylindrical microchannel in said polymer body, said cylindrical microchannel having a diameter between approximately 40 μ

m and 250 μ

m, inclusive.

21. The microfluidic device of claim 20, wherein said cylindrical microchannel has a diameter between approximately 57 μ

m and 125 μ

m, inclusive.

22. The microfluidic device of claim 20, wherein said polymer body includes polydimethylsiloxane (PDMS).

23. The microfluidic device of claim 20, wherein said polymer body includes a biopolymer selected from a group consisting of chitosan, collagen, gelatin, agarose, chitin, polyhydroxyalkanoates, pullan, starch (amylose amylopectin), cellulose, hyaluronic acid, and related biopolymers, or a combination thereof.

24. The microfluidic device of claim 20, wherein said polymer body includes a silk biopolymer.

25. The microfluidic device of claim 20, wherein said polymer body includes an optical element on a surface thereof.

26. The microfluidic device of claim 20, wherein said optical element is at least one of a lens, a microlens array, an optical grating, a pattern generator, a beam reshaper, a mirror blank, and a glass slide.

27. The microfluidic device of claim 20, wherein said polymer body includes a doping agent.

28. The microfluidic device of claim 27, wherein said doping agent is selected from a group consisting of red blood cells, horseradish peroxidase, and phenolsulfonphthalein, or a combination thereof.

29. The microfluidic device of claim 27, wherein said doping agent is selected from a group consisting of a nucleic acid, a dye, a cell, an antibody, enzymes, for example, peroxidase, lipase, amylose, organophosphate dehydrogenase, ligases, restriction endonucleases, ribonucleases, DNA polymerases, glucose oxidase, laccase, cells, viruses, proteins, peptides, small molecules, drugs, dyes, amino acids, vitamins, antixoxidants, DNA, RNA, RNAi, lipids, nucleotides, aptamers, carbohydrates, chromophores, light emitting organic compounds such as luciferin, carotenes and light emitting inorganic compounds, chemical dyes, antibiotics, antifungals, antivirals, light harvesting compounds such as chlorophyll, bacteriorhodopsin, protorhodopsin, and porphyrins and related electronically active compounds, or a combination thereof.

30. An optofluidic spectrometer comprising:

a polymer body;

a diffraction grating integrated with said polymer body; and

at least one cylindrical microchannel in said polymer body, said cylindrical microchannel having a diameter between approximately 40 μ

m and 250 μ

m, inclusive, and behind said diffraction grating on said polymer body.

31. The optofluidic spectrometer of claim 30, wherein said polymer body is a siloxane polymer chip.

32. The optofluidic spectrometer of claim 31, wherein said siloxane polymer chip includes polydimethylsiloxane (PDMS).

33. The optofluidic spectrometer of claim 30, wherein said polymer body is a biopolymer.

34. The optofluidic spectrometer of claim 33, wherein said biopolymer is silk.

35. A method of probing absorption of a fluid in a microfluidic channel comprising:

transmitting light through a polymer body, wherein said polymer body includes said microfluidic channel containing said fluid and a diffraction grating;

absorbing at least one wavelength of said light in said fluid;

diffracting said light with said diffraction grating;

analyzing said diffracted light for transmitted power as a function of wavelength with a slit; and

characterizing said fluid based upon said analysis of said diffracted light.