METHODS FOR RAPID IDENTIFICATION AND QUANTITATION OF NUCLEIC ACID VARIANTS
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Abstract
There is a need for nucleic acid analysis which is both specific and rapid, and in which no nucleic acid sequencing is required. The present invention addresses this need, among others by providing a method of nucleic acid amplification of overlapping sub-segments of a nucleic acid followed by molecular mass measurement of resulting amplification products by mass spectrometry, and determination of the base compositions of the amplification products.
180 Citations
54 Claims
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1-36. -36. (canceled)
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37. A system, comprising:
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at least one mass spectrometer configured to measure molecular masses of overlapping amplification products representing sub-segments of a nucleic acid; and
,at least one computer configured to determine base compositions of said overlapping amplification products from said molecular masses and to identify a source of said nucleic acid from said base compositions. - View Dependent Claims (38, 39, 40, 41, 42, 43)
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44. A method of analyzing data using a computer, the method comprising:
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receiving data comprising molecular masses of overlapping amplification products representing sub-segments of a nucleic acid at said computer; determining base compositions of said overlapping amplification products from said molecular masses; and
,identifying a source of said nucleic acid from said base compositions, thereby analyzing said data using said computer. - View Dependent Claims (45, 46, 47, 48)
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49. A base composition analysis method, the method comprising:
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(a) generating at least two overlapping amplification products from at least two sub-segments of a nucleic acid; (b) measuring molecular masses of said overlapping amplification products using a mass spectrometer; (c) converting said measured molecular masses to base compositions; and
,(d) comparing said base compositions with base composition data for said nucleic acid to identify a source of the nucleic acid. - View Dependent Claims (50, 51, 52, 53, 54)
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Specification