SSB-POLYMERASE FUSION PROTEINS
First Claim
Patent Images
1. An isolated or purified single stranded binding protein (SSB)-nucleic acid polymerase fusion protein.
0 Assignments
0 Petitions
Accused Products
Abstract
Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
79 Citations
25 Claims
- 1. An isolated or purified single stranded binding protein (SSB)-nucleic acid polymerase fusion protein.
- 8. A nucleic acid molecule encoding an SSB-nucleic acid polymerase fusion protein.
-
17. A nucleic acid molecule encoding a Sulfolobus sofataricus (Sso) SSB-Thermococcus zilligi (Tzi) DNA polymerase fusion protein.
-
18. A method of increasing the yield of a polymerase reaction on a target nucleic acid comprising:
-
(a) contacting a target nucleic acid molecule with a primer which specifically hybridizes thereto, and an isolated or purified single-stranded binding protein (SSB)-nucleic acid polymerase fusion protein which binds to single stranded DNA and enhances the processivity of said nucleic acid polymerase compared to said polymerase which does not have a SSB fused thereto; and (b) incubating the resulting mixture under conditions whereby said primer is extended by said polymerase. - View Dependent Claims (19, 20, 21, 22, 23, 24)
-
-
25. An isolated or purified Sulfolobus sofataricus (Sso) SSB-Thermococcus zilligi (Tzi) DNA polymerase fusion protein
Specification