BIOREACTOR JACKET

• US 20100075405A1
• Filed: 02/14/2008
• Published: 03/25/2010
• Est. Priority Date: 02/15/2007
• Status: Abandoned Application

• Current Assignee: Broadley James Corporation
• Original Assignee: Broadley James Corporation
• Inventors: Broadley, Scott T., Benton, Patricia R.
• Application Number:
  US12/525,300
• Publication Number:
  US 20100075405A1
• Time in Patent Office: Days
• US Class Current: 435/286.100
• CPC Class Codes: C12M 23/14 Bags
• CPC Class Codes: C12M 23/28 disposable or single use
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12M 41/22 in contact with the bioreactor walls
• CPC Class Codes: Y10T 137/0324 With control of flow by a condition or characteristic of a fluid

Abstract

A bioreactor system (10) is provided for controlling temperature of bioreactor media during processing. The system (10) includes a flexible bag (102) having an inner wall (104) defining an inner volume (105) for holding the cell culture media and an outer wall (106) surrounding at least a portion of the inner wall (104). Together, the inner wall (104) and the outer wall (106) form a jacket volume (107) which can hold a temperature control fluid.

Claims

1. A bioreactor bag for affecting the temperature of media held therein, the bag comprising:

a flexible inner wall defining an inner volume for holding the media; and

a flexible outer wall surrounding at least a portion of the inner wall, the inner wall and the outer wall together defining an outer volume for holding a temperature control fluid therein, the outer volume being separate from the inner volume.

2. The bioreactor bag of claim 1, further comprising:

an entrance port configured to receive the temperature control fluid into the outer volume; and

an exit port configured to exhaust the fluid from the outer volume.

3. The bioreactor bag of claim 1, wherein the fluid is a liquid.

4. The bioreactor bag claim 1, wherein the fluid is a gas.

5. The bioreactor bag of claim 1, wherein the inner volume of the bioreactor bag has a capacity of between about 100 milliliters and about 5000 liters.

6. The bioreactor bag of claim 1, wherein the outer volume of the bioreactor has a capacity between about 1 percent and about 50 percent of the inner volume capacity.

7. The bioreactor bag of claim 1, wherein the inner wall and the outer wall comprise plastic.

8. The bioreactor bag of claim 1, wherein the outer volume entirely encircles the inner volume when the inner volume is at least partially filled with media and the outer volume is at least partially filled with fluid.

9. The bioreactor bag of claim 1, wherein the outer volume extends below the inner volume when the inner volume is at least partially filled with media and the outer volume is at least partially filled with fluid.

10. A bioreactor system comprising the bioreactor bag of claim 1, the system further comprising a temperature control module configured to affect the temperature of the fluid in the outer volume.

11. The bioreactor system of claim 10, wherein the temperature control module comprises a heat exchanger disposed outside the outer volume.

12. The bioreactor system of claim 10, wherein the temperature control module comprises a temperature adjusting element disposed in contact with the outer volume.

13. The bioreactor system of claim 12, wherein the temperature adjusting element extends into the outer volume.

14. The bioreactor system of claim 12, wherein the temperature adjusting element is a resistive heating element.

15. The bioreactor system of claim 12, wherein the temperature adjusting element is a cold finger.

16. The bioreactor system of claim 12, wherein the temperature adjusting element is a hot finger.

17. A bioreactor system for affecting the temperature of media, the system comprising:

a flexible jacket bag configured to hold a temperature control fluid and configured to at least partially surround a bioreactor bag when the bioreactor bag is at least partially filled with media, the jacket bag comprising

an inlet port configured to receive the temperature control fluid into the jacket bag from a temperature control mechanism, and

an outlet port configured to exhaust the fluid from the jacket bag.

18. The bioreactor system of claim 17, wherein an interior wall of the jacket bag is configured to contact an outer wall of the bioreactor bag when the jacket bag is at least partially filled with the temperature control media.

19. The bioreactor system of claim 17, further comprising the bioreactor bag.

20. The bioreactor system of claim 17, further comprising the temperature control mechanism.

21. A bioreactor bag comprising:

first flexible means for holding media in a sterile environment; and

second flexible means for holding a temperature control fluid separate from but adjacent to the media, the second flexible means at least partially surrounding the first flexible means, the second flexible means comprising means for receiving and exhausting the temperature control fluid.

22. A method of regulating the temperature of media during processing, the method comprising:

providing a bioreactor bag comprising a flexible inner wall defining an inner volume for holding the media and a flexible outer wall surrounding at least a portion of the inner wall, the inner wall and the outer wall together defining an outer volume for holding a temperature control fluid therein, the outer volume being separate from the inner volume; and

filling the outer volume at least partially with the temperature control fluid.

23. The method of claim 22, further comprising adjusting the temperature of the temperature control fluid.

24. A method of regulating temperature in a flexible bioreactor bag having a first flexible surface configured to hold media and a second flexible surface surrounding at least a portion of the first surface, the first surface and the second surface defining a cavity therebetween, the method comprising:

providing fluid into the cavity formed between the first surface of the bioreactor bag and the second surface of the bioreactor bag through an entrance port;

exhausting fluid from the cavity through an exit port; and

affecting the temperature of media held by the first flexible surface by the temperature of the fluid provided to the cavity.

25. The method of claim 24, further comprising controlling the temperature of the fluid provided to the flexible bioreactor bag.

26. The method of claim 25, the method further comprising:

sensing the temperature of media held by the first flexible surface; and

controlling the temperature of the fluid provided to the flexible bioreactor bag based on the sensed temperature.