SAMPLE PROCESSING UNITS, SYSTEMS, AND RELATED METHODS

• US 20100075430A1
• Filed: 09/16/2009
• Published: 03/25/2010
• Est. Priority Date: 09/16/2008
• Status: Active Grant

• Current Assignee: Ibis Biosciences Incorporated
• Original Assignee: Ibis Biosciences Incorporated
• Inventors: Hofstadler, Steven A., Drader, Jared J., Gutierrez, Jose R., Bare, Rex O., Gleason, Paul J., Smith, Jeffrey C., Miller, Robert D.
• Granted Patent: US 8,148,163 B2
• Time in Patent Office: Days
• US Class Current: 436/94
• CPC Class Codes: G01N 1/02 Devices for withdrawing samples sampling of foundation soil E02D1/04; collecting or conveying radioactive samples G01T7/00, e.g. G01T7/02, G01T7/08
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: Y10T 436/14 Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
• CPC Class Codes: Y10T 436/142222 Hetero-O [e.g., ascorbic acid, etc.]
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided.

Claims

1. A sample processing unit, comprising:

at least one container configured to contain at least one sample comprising at least one magnetically responsive particle;

at least one magnet that generates, or is configured to generate, at least one magnetic field, which magnet is in a substantially fixed position relative to the container;

at least one conveyance mechanism configured to convey the container between at least first and second positions, wherein at least the first position is within magnetic communication with the magnet when the magnet generates the magnetic field; and

,

at least one rotational mechanism operably connected to the container, which rotational mechanism is configured to rotate the container when the container is in at least the second position.

2. The sample processing unit of claim 1, wherein the container comprises a cuvette.

3. The sample processing unit of claim 1, wherein the magnet comprises a permanent magnet.

4. The sample processing unit of claim 1, wherein the conveyance mechanism comprises at least one motor.

5. The sample processing unit of claim 1, wherein the conveyance mechanism is configured to rotate the container between the first and second positions.

6. The sample processing unit of claim 1, wherein the conveyance mechanism is configured to slide the container between the first and second positions.

7. The sample processing unit of claim 1, wherein the rotational mechanism is configured to rotate the container in at least one pulsed mode, during which a substantial portion of the time of rotation, a rate of rotation of the container exceeds a rate of rotation of the sample when the container contains the sample such that the sample is sheared away from a surface of the container.

8. The sample processing unit of claim 1, wherein the rotational mechanism is configured to rotate the container in at least one oscillating motion.

9. The sample processing unit of claim 1, comprising at least one mounting bracket that is operably connected to one or more of:

the container, the magnet, the conveyance mechanism, or the rotational mechanism.

10. The sample processing unit of claim 1, wherein the conveyance mechanism comprises at least one support member operably connected to the container and/or to the rotational mechanism.

11. The sample processing unit of claim 10, wherein the support member is configured to slide between the first and second positions.

12. The sample processing unit of claim 10, wherein the support member is configured to rotate between the first and second positions.

13. A sample processing unit, comprising:

at least one cuvette configured to contain at least one sample comprising at least one magnetically responsive particle;

at least one magnet that generates, or is configured to generate, at least one magnetic field, which magnet is in a substantially fixed position;

at least a first motor operably connected to the cuvette, which first motor is configured to rotate the cuvette around a central longitudinal axis of the cuvette;

at least one support member operably connected to the first motor; and

,

at least a second motor operably connected to the support member, which second motor is configured to rotate the cuvette between at least first and second positions, wherein at least the first position is within magnetic communication with the magnet when the magnet generates the magnetic field.

14. The sample processing unit of claim 13, wherein the magnet comprises a permanent magnet.

15. The sample processing unit of claim 13, comprising two or more magnets disposed proximal to a receiving space, wherein the cuvette is located at least partially within the receiving space when the cuvette is in the first position.

16. The sample processing unit of claim 13, wherein the first motor is configured to rotate the cuvette in at least one pulsed mode, during which a substantial portion of the time of rotation, a rate of rotation of the cuvette exceeds a rate of rotation of the sample when the cuvette contains the sample such that the sample is sheared away from a surface of the cuvette.

17. The sample processing unit of claim 13, wherein the first motor is configured to rotate the cuvette in at least one oscillating motion.

18. The sample processing unit of claim 13, wherein the first motor comprises a stepper motor.

19. The sample processing unit of claim 13, wherein the support member comprises a swing arm.

20. The sample processing unit of claim 13, wherein the second motor comprises a brushless direct current motor.

21. The sample processing unit of claim 13, comprising circuitry configured to control the first and second motors.

22. The sample processing unit of claim 13, wherein the support member comprises a first end and a second end, wherein the cuvette is retained at or proximal to the first end of the support member, and wherein the second motor is operably connected to the support member at or proximal to the second end of the support member.

23. The sample processing unit of claim 22, wherein the support member is configured to rotate at least partially around a rotational axis extending through the second end of the support member.

24. The sample processing unit of claim 23, comprising a pin fixedly coupled to the second end of the support member and aligned with the rotational axis, wherein the pin is operably coupled to the second motor.

25. The sample processing unit of claim 13, comprising a mounting bracket, wherein the support member is operably connected to the mounting bracket.

26. The sample processing unit of claim 25, wherein the magnet is operably connected to the mounting bracket.

27. A sample processing system, comprising:

at least one sample processing unit that comprises;

at least one cuvette configured to contain at least one sample comprising at least one magnetically responsive particle;

at least one magnet that generates, or is configured to generate, at least one magnetic field, which magnet is in a substantially fixed position;

at least a first motor operably connected to the cuvette, which first motor is configured to rotate the cuvette around a central longitudinal axis of the cuvette;

at least one support member operably connected to the first motor; and

at least a second motor operably connected to the support member, which second motor is configured to rotate the cuvette between at least first and second positions, wherein at least the first position is within magnetic communication with the magnet when the magnet generates the magnetic field;

at least one carrier mechanism operably connected to the sample processing unit, which carrier mechanism is configured to move the sample processing unit to one or more locations;

at least one material transfer component configured to transfer material to and/or from the cuvette; and

,

at least one controller operably connected to the sample processing unit, the carrier mechanism, and/or the material transfer component, which controller is configured to effect one or more of;

the magnet to generate the magnetic field, the first motor to rotate the cuvette, the second motor to rotate the cuvette between the first and second positions, the carrier mechanism to move the sample processing unit to the one or more locations, or the material transfer component to transfer the material to and/or from the cuvette.

28. The sample processing system of claim 27, wherein the controller is configured to effect the first motor to rotate the cuvette in one or more selectable modes.

29. The sample processing system of claim 27, wherein the controller is configured to effect the first motor to rotate the cuvette in at least one pulsed mode, during which a substantial portion of the time of rotation, a rate of rotation of the cuvette exceeds a rate of rotation of the sample when the cuvette contains the sample such that the sample is sheared away from a surface of the cuvette.

30. The sample processing system of claim 27, wherein the controller is configured to effect the first motor to rotate the cuvette in at least one oscillating motion.

31. The sample processing system of claim 27, wherein the carrier mechanism comprises a carousel that is configured to rotate the sample processing unit to the one or more locations.

32. The sample processing system of claim 27, wherein the carrier mechanism comprises a conveyor track that is configured to convey the sample processing unit to the one or more locations.

33. The sample processing system of claim 27, wherein the material transfer component comprises one or more needles.

34. The sample processing system of claim 27, wherein the material transfer component is configured to transfer fluidic material.

35. The sample processing system of claim 27, wherein the material transfer component comprises one or more of:

a sample input gantry head, a sample wash station, a sample output gantry head, or a cuvette wash station.

36. The sample processing system of claim 27, wherein the carrier mechanism comprises a plurality of sample processing units.

37. The sample processing system of claim 36, wherein the material transfer component comprises a manifold that is configured to transfer material to and/or from the cuvettes of at least two sample processing units substantially simultaneously.

38. The sample processing system of claim 27, comprising at least one detector configured to detect one or more detectable signals of or from one or more sample components.

39. The sample processing system of claim 38, wherein the detector is within sensory communication with the cuvette when the carrier mechanism moves the sample processing unit to at least one of the locations.

40. The sample processing system of claim 38, wherein the material transfer component is configured to transfer the material from the cuvette to the detector.

41. The sample processing system of claim 38, wherein the controller is operably connected to the detector, which controller is configured to effect the detector to detect the detectable signals of or from the sample components.

42. The sample processing system of claim 38, wherein the detector comprises a mass spectrometer.

43. The sample processing system of claim 42, wherein the mass spectrometer comprises an electrospray ionization time-of-flight mass spectrometer.

44. A method of processing a sample, the method comprising:

(a) providing at least one sample processing unit that comprises;

at least one cuvette that contains at least one sample comprising at least one magnetically responsive particle comprising at least one captured first component;

at least one magnet that is in a substantially fixed position;

at least a first motor operably connected to the cuvette, which first motor is configured to rotate the cuvette around a central longitudinal axis of the cuvette;

at least one support member operably connected to the first motor; and

at least a second motor operably connected to the support member, which second motor is configured to rotate the cuvette between at least first and second positions, wherein the magnet is capable of magnetically communicating with the cuvette when the cuvette is at least in the first position;

(b) moving the cuvette into the first position using the second motor such that a magnetic field generated by the magnet causes the magnetically responsive particle to move and/or be retained proximal to a surface of the cuvette; and

,

(c) removing at least a second component from the cuvette, thereby processing the sample.

45. The method of claim 44, comprising adding the sample and/or the magnetically responsive particle to the cuvette prior to (a) when the cuvette is in the second position.

46. The method of claim 44, comprising adding at least one wash reagent to the cuvette.

47. The method of claim 44, wherein the first component comprises a biopolymer.

48. The method of claim 44, wherein the magnet comprises a permanent magnet.

49. The method of claim 44, wherein the magnet comprises an electromagnet and wherein the method comprises generating the magnetic field prior to or during (b).

50. The method of claim 44, wherein a carrier mechanism comprises the sample processing unit and wherein the method comprises moving the sample processing unit to one or more locations.

51. The method of claim 44, wherein the magnetically responsive particle comprises an anion exchange resin.

52. The method of claim 51, wherein the magnetically responsive particle comprises at least one biopolymer capture reagent.

53. The method of claim 52, wherein the biopolymer capture reagent comprises at least one anion exchange functional group.

54. The method of claim 53, wherein the anion exchange functional group comprises a pK_a value of 9.0 or greater.

55. The method of claim 53, wherein the anion exchange functional group is selected from the group consisting of:

a primary amine, a second amine, a tertiary amine, a quaternary amine, a polyethyleneimine, a charged aromatic amine, a diethylaminomethyl, and a diethylaminoethyl.

56. The method of claim 44, comprising rotating the cuvette using the first motor such that sample components mix with one another.

57. The method of claim 56, comprising rotating the cuvette when the cuvette is in the second position.

58. The method of claim 56, comprising rotating the cuvette in at least one pulsed mode, during which a substantial portion of the time of rotation, a rate of rotation of the cuvette exceeds a rate of rotation of the sample such that the sample is sheared away from a surface of the cuvette.

59. The method of claim 56, comprising rotating the cuvette in at least one oscillating motion.

60. The method of claim 44, comprising detecting at least one detectable signal of or from the sample.

61. The method of claim 60, comprising detecting a molecular mass of the first component.

62. The method of claim 61, wherein the molecular mass is detected using a mass spectrometer.

63. The method of claim 61, wherein the first component comprises a nucleic acid and wherein the method comprises correlating the molecular mass of the nucleic acid with a base composition and/or an identity of the nucleic acid.