BIOLOGICAL BAR CODE

• US 20100075858A1
• Filed: 05/22/2009
• Published: 03/25/2010
• Est. Priority Date: 04/29/2003
• Status: Abandoned Application

• Current Assignee: GenVault Corp. (Thermo Fisher Scientific Incorporated)
• Original Assignee: GenVault Corp. (Thermo Fisher Scientific Incorporated)
• Inventors: WONG, David, IBARRA, Rafael, SAGHBINI, Michael, JAFFE, Syrus M., EGGERS, Mitchell D., SADLER, John, HOGAN, Michael, DAVIS, James C.
• Application Number:
  US12/471,321
• Publication Number:
  US 20100075858A1
• Time in Patent Office: Days
• US Class Current: 506/4
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 2563/185 Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
• CPC Class Codes: C12Q 2565/514 characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

Abstract

The invention provides coding compositions comprising mixtures of coding oligonucleotides and methods of using such compositions to code samples. The compositions and methods are useful for identifying, verifying, or authenticating any type of sample, whether the sample is biological or non-biological.

Claims

1. A coded storage package comprising:

a container containing a subset of coding oligonucleotides from a predetermined pool of coding oligonucleotides, and

an identifying indicia attached to said container

wherein the coding oligonucleotides of said pool each comprise a unique identifier sequence, wherein the combination of oligonucleotides represents the presence and absence of oligonucleotides from said pool and such representation constitutes a code, and wherein said identifying indicia identifies the code represented by said subset of coding oligonucleotides.

2. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset has a non-naturally occurring sequence.

3. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset comprises one or more modified bases.

4. The coded storage package of claim 1, wherein said subset of coding oligonucleotides comprises 2, 3, 4, 5 or more coding oligonucleotides from said pool.

5. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset comprises a detection sequence.

6. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset comprises a 5′

leader sequence, wherein said leader sequence is not part of an identifier sequence or a detection sequence.

7. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset comprises a detection sequence and a 5′

leader sequence.

8. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset is 40 to 70 bases long.

9. The coded storage package of claim 1, wherein each coding oligonucleotide of said subset is labeled.

10. The coded storage package of claim 1, further comprising a plurality of said containers.

11. The coded storage package of claim 10, wherein the plurality of said containers are wells in a multi-well plate.

12. The coded storage package of claim 10, wherein each container of said plurality has the same code.

13. The coded storage package of claim 10, wherein the plurality of said containers is divided into 2, 3, 4, 5, 6 or more groups, and wherein each container in the same group has the same code.

14. The coded storage package of claim 1, wherein said container comprises a sample node, and wherein said sample node carries said subset of coding oligonucleotides.

15. The coded storage package of claim 14, wherein said sample node comprises a sample support medium, and wherein said sample support medium carries said subset of coding oligonucleotides.

16. The coded storage package of claim 14, wherein said sample node comprises a porous material.

17. The coded storage package of claim 14, wherein said sample node comprises cellulose or an elastomeric foam.

18. The coded storage package of claim 1, further comprising a biological sample.

19. The coded storage package of claim 18, wherein each coding oligonucleotide of said subset is incapable of specifically hybridizing to said biological sample or to pathogens associated with said biological sample.

20. An archive of biological samples, wherein each sample is stored in a container of claim 1.

21. A method for coding a sample comprising adding said sample to a container of claim 1.

22. A method for coding a sample comprising:

adding a subset of coding oligonucleotides to said sample,

wherein said subset is from a predetermined pool of coding oligonucleotides, wherein the coding oligonucleotides of said pool are different from each other, and wherein the combination of oligonucleotides represents the presence and absence of oligonucleotides from said pool and such representation constitutes a code.

23. The method of claim 22, further comprising selecting said subset of coding oligonucleotides from said predetermined pool of coding oligonucleotides prior to said adding.

24. A coded sample made according to the method of claim 22.

25. A method of decoding a coded sample, wherein the code comprises a subset of coding oligonucleotides from a predetermined pool of coding oligonucleotides, and wherein the coding oligonucleotides of said pool are different from each other, the method comprising:

detecting one or more coding oligonucleotides of said pool in said sample,

wherein a collective result of the presence and absence of said one or more oligonucleotides of said pool in said sample is indicative of a code associated with said sample.

26. The method of claim 25, comprising detecting the presence and absence of each coding oligonucleotide of said pool in said sample.

27. The method of claim 25, further comprising determining the code of said coded sample based upon said detecting.

28. The method of claim 25, wherein said detecting comprises contacting each of said one or more coding oligonucleotides with an identifier oligonucleotide corresponding to each coding oligonucleotide of said pool, wherein each identifier oligonucleotide is bound to an addressable array.

29. The method of claim 25, wherein said detecting comprises contacting each of said one or more coding oligonucleotides with a detection oligonucleotide, and an identifier oligonucleotide corresponding to each coding oligonucleotide of said pool, wherein each identifier oligonucleotide is bound to an addressable array.

30. The method of claim 25, wherein said detecting comprises contacting each of said one or more coding oligonucleotides with an identifier oligonucleotide corresponding to each coding oligonucleotide of said pool, wherein each identifier oligonucleotide is indirectly bound to an addressable array.

31. The method of claim 25, wherein said detecting comprises contacting each of said one or more coding oligonucleotides with a detection oligonucleotide and an identifier oligonucleotide corresponding to each coding oligonucleotide of said pool, wherein each identifier oligonucleotide is indirectly bound to an addressable array.

32. The method of claim 25, wherein said detecting comprises contacting each of said one or more coding oligonucleotides with a detection oligonucleotide, a labeling oligonucleotide, and an identifier oligonucleotide corresponding to each coding oligonucleotide of said predetermined pool, wherein each identifier oligonucleotide is indirectly bound to an addressable array, and wherein each detection oligonucleotide is bound to a labeling oligonucleotide.

33. The method of claim 25, wherein said detecting comprising detecting a label incorporated into each of said one or more coding oligonucleotides.

34. The method of claim 29, wherein said detecting comprises detecting a label associated with said detection oligonucleotide.

35. The method of claim 31, wherein said detecting comprises detecting a label associated with said detection oligonucleotide.

36. The method of claim 32, wherein said detecting comprises detecting a label associated with said detection oligonucleotide.

37. A kit comprising:

a container containing a substrate for biological molecule storage and a subset of coding oligonucleotides from a predetermined pool of coding oligonucleotides, wherein the oligonucleotides of said pool are different from each other, and wherein the combination of oligonucleotides represents the presence and absence of oligonucleotides from said pool and such representation constitutes a code.

38. The kit of claim 37, further comprising identifying indicia, wherein said identifying indicia identifies the code represented by said subset of coding oligonucleotides.

39. The kit of claim 37, further comprising a set of identifier oligonucleotides, wherein said set of identifier oligonucleotides can be used to decode the code contained in said container.

40. The kit of claim 37, further comprising a set of identifier oligonucleotides and a corresponding set of secondary identifier oligonucleotides, wherein said set of identifier oligonucleotides and said set of corresponding secondary identifier oligonucleotides can be used to decode the code contained in said container.

41. The kit of claim 37, further comprising a set of identifier oligonucleotides and at least one detection oligonucleotide, wherein said set of identifier oligonucleotides and said at least one detection oligonucleotide can be used to decode the code contained in said container.

42. The kit of claim 37, further comprising a set of identifier oligonucleotides, a set of corresponding secondary identifier oligonucleotides and at least one detection oligonucleotide, wherein said set of identifier oligonucleotides, said set of secondary identifier oligonucleotides and said at least one detection oligonucleotide can be used to decode the code contained in said container.

43. The kit of claim 37, further comprising a set of identifier oligonucleotides, a set of corresponding secondary identifier oligonucleotides, at least one detection oligonucleotide and corresponding signaling oligonucleotides, wherein said set of identifier oligonucleotides, said set of secondary identifier oligonucleotides, said at least one detection oligonucleotide and corresponding labeling oligonucleotides can be used to decode the code contained in said container.

44. The kit of claim 37, wherein said substrate is suitable for long-term storage of biological molecules.