Transferases, Epimerases, Polynucleotides Encoding These and Uses Thereof
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Abstract
The invention provides compositions and methods for modulating GDP-L-Galactose Guanyltransferase (also known as GDP-L-Galactose phosphorylase) activity; and/or GDP-D-Mannose epimerase activity; and/or ascorbate content in plants. The invention provides plants and plant cells with increased GDP-L-Galactose Guanyltransferase activity; and/or GDP-D-Mannose epimerase activity. The invention provides plants and plant cells with increased ascorbate content as a result of: over-expression of GDP-L-Galactose Guanyltransferase; over-expression of GDP-D-Mannose epimerase; or in particular over-expression of a combination of GDP-L-Galactose Guanyltransferase and GDP-D-Mannose epimerase.
38 Citations
93 Claims
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1-62. -62. (canceled)
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63. A method for producing a plant cell or plant with increased ascorbate, the method comprising transformation of a plant cell or plant with a polynucleotide encoding a polypeptide with the amino acid sequence of any one of SEQ ID NO:
- 1 to 11, or a variant of the polypeptide, wherein the variant has the activity of an GDP-L-Galactose Guanyltransferase.
- View Dependent Claims (64, 65, 66, 67, 68, 92)
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69. A method for producing a plant cell or plant with increased ascorbate content, the method comprising transformation of a plant cell or plant with:
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a) a polynucleotide encoding a GDP-D-Mannose epimerase; and b) a polynucleotide encoding a GDP-L-Galactose Guanyltransferase. - View Dependent Claims (71, 72, 73, 74, 75, 76, 77)
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70. The method of claim 697 in which the GDP-D-Mannose epimerase comprises the amino acid sequence AADMGGMGFIQSNHSVI (SEQ ID NO:
- 36).
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78. An isolated polynucleotide encoding a polypeptide comprising a sequence with at least 78% identity to the sequence of any one of SEQ ID NO:
- 1 to 7, wherein the polypeptide is a GDP-L-Galactose Guanyltransferase.
- View Dependent Claims (79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 93)
Specification