Sample analyzer, and a method for analyzing a sample

• US 20100079762A1
• Filed: 09/28/2009
• Published: 04/01/2010
• Est. Priority Date: 09/30/2008
• Status: Abandoned Application

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Kusuzawa, Hideo
• Application Number:
  US12/586,819
• Publication Number:
  US 20100079762A1
• Time in Patent Office: Days
• US Class Current: 356/442
• CPC Class Codes: G01N 15/1425 using an analyser being characterised by its control arrangement
• CPC Class Codes: G01N 15/1434 Optical arrangements
• CPC Class Codes: G01N 15/147 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/1497 Particle shape

Abstract

A sample analyzer comprising a flow cell for forming a sample flow, a light source for irradiating the sample flow in the flow cell with near ultraviolet light, and an imaging section for taking an image of a particle contained in the sample flow irradiated with the near ultraviolet light by the light source is disclosed. A method for analyzing a sample is also disclosed.

Claims

1. A sample analyzer comprising:

a flow cell for forming a sample flow;

a light source for irradiating the sample flow in the flow cell with near ultraviolet light; and

an imaging section for taking an image of a particle contained in the sample flow irradiated with the near ultraviolet light by the light source.

2. The sample analyzer according to claim 1, further comprising a drive circuit for controlling the light source to perform pulse emission.

3. The sample analyzer according to claim 1, further comprising:

a particle detector for detecting the particle contained in the sample flow in the flow cell; and

an imaging controller for, when the particle is detected by the particle detector, controlling the imaging section to take an image of the detected particle.

4. The sample analyzer according to claim 3, wherein

the particle detector comprises:

a second light source for irradiating the sample flow in the flow cell with light having a wavelength longer than that of the near ultraviolet light emitted by the light source; and

a light receiver for receiving fluorescence from a particle that is excited by the irradiation light of the second light source.

5. The sample analyzer according to claim 4, wherein

the light source, the second light source, and the particle detector are configured to prevent a particle from being detected due to fluorescence emitted from the particle that is excited by the irradiation with the near ultraviolet light from the light source.

6. The sample analyzer according to claim 1, further comprising:

a display; and

a display controller for controlling the display to display thereon the image of the particle that is taken by the imaging section.

7. The sample analyzer according to claim 6, wherein

when the image of the particle is taken by the imaging section, the display controller causes the display to display thereon the image of the particle.

8. The sample analyzer according to claim 1, further comprising a light selector positioned between the flow cell and the imaging section, the light selector being configured to select near ultraviolet light and allow the near ultraviolet light to pass therethrough.

9. The sample analyzer according to claim 1, further comprising:

an irradiation lens system positioned between the light source and the flow cell; and

an imaging lens system positioned between the flow cell and the imaging section, wherein

a numerical aperture of the irradiation lens system is set so as to be less than a numerical aperture of the imaging lens system.

10. The sample analyzer according to claim 1, further comprising a sample preparing section for preparing a sample using urine, and for supplying the sample to the flow cell, wherein

the imaging section is configured to take an image of a urine formed element as the particle.

11. The sample analyzer according to claim 3, wherein

when a deformed erythrocyte is detected by the particle detector, the imaging controller controls the imaging section to take an image of the deformed erythrocyte.

12. The sample analyzer according to claim 1, wherein

the light source is configured to emit, as the near ultraviolet light, light with a peak wavelength between 200 and 400 nm.

13. The sample analyzer according to claim 1, further comprising an analyzing section for analyzing the taken image, and for obtaining an analysis result of the particle contained in the sample.

14. A method for analyzing a sample comprising:

supplying a sample to a flow cell to form a sample flow;

irradiating the sample flow with near ultraviolet light; and

taking an image of a particle contained in the sample flow irradiated with the near ultraviolet light.

15. The method according to claim 14, wherein the irradiating is performed by irradiation with light by pulse emission.

16. The method according to claim 14, further comprising detecting the particle contained in the sample flow, wherein

when the particle is detected, the image taking step is performed by taking the image of the detected particle.

17. The method according to claim 16, wherein

the particle detecting is performed by receiving fluorescence from a particle that is excited by irradiating the sample flow in the flow cell with light having a wavelength longer than that of the near ultraviolet light.

18. The method according to claim 14, further comprising displaying the taken image of the particle.

19. The method according to claim 17, wherein

the particle detecting comprises detecting a deformed erythrocyte, and

the image taking comprises taking an image of the detected deformed erythrocyte.

20. The method according to claim 14, further comprising analyzing the taken image and obtaining an analysis result of the particle contained in the sample.