POLYMERIC FILMS TO ENHANCE CELL GROWTH

• US 20100080987A1
• Filed: 09/28/2009
• Published: 04/01/2010
• Est. Priority Date: 09/26/2008
• Status: Abandoned Application

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Xu, Hao, Timmons, Richard Brendon, Bhattacharyya, Dhiman, Nguyen, Kytai Troung
• Application Number:
  US12/568,494
• Publication Number:
  US 20100080987A1
• Time in Patent Office: Days
• US Class Current: 428/336
• CPC Class Codes: C08F 2/46 Polymerisation initiated by wave energy or particle radiation
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: Y10T 428/265 1 mil or less

Abstract

The present invention provides a method for creating surface-tailored polymer coating for improved cells proliferation by converting one or more carbonaceous monomers each comprising one or more functional groups to a monomer vapor; contacting the monomer vapor and a substrate; and igniting a radio frequency plasma glow discharge to polymerize the monomer vapor to form surface-tailored polymer coating on the substrate at a thicknesses of between 25 and 300 nm and has a functional group surface density of between 3 and 9%, wherein the surface-tailored polymer coating enhances cell proliferation.

Claims

1. A method for creating surface-tailored polymer coating for improved cells proliferation comprising the steps of:

converting one or more carbonaceous monomers comprising one or more functional groups to a monomer vapor;

contacting the monomer vapor and a substrate; and

igniting a radio frequency plasma glow discharge to polymerize the monomer vapor to form surface-tailored polymer coating on the substrate at a thicknesses of between 25 and 300 nm and has a functional group surface density of between 3 and 9%, wherein the surface-tailored polymer coating enhances cell proliferation.

2. The method of claim 1, wherein the one or more carbonaceous monomers comprise one or more vinyl acetic acid monomers, one or more acetic acid monomers, one or more ethylene acetic acid monomers or a combination thereof.

3. The method of claim 1, further comprising one or more carbonaceous monomers without functional groups to control the functional group surface density.

4. The method of claim 1, wherein the radio frequency plasma glow discharge is a continuous-wave radio frequency plasma glow discharge.

5. The method of claim 1, wherein the radio frequency plasma glow discharge is a pulsed-wave radio frequency plasma glow discharge.

6. The method of claim 5, wherein the pulsed-wave radio frequency plasma glow discharge has a duty cycles of 2 ms on and 30 ms off or 10 ms on and 30 ms off.

7. The method of claim 5, wherein the radio frequency plasma glow discharge has an average power input between 9.4 and 37.5 W.

8. The method of claim 1, wherein the substrate comprises a tissue culture plate.

9. The method of claim 1, wherein the thicknesses is between 25 and 200 nm.

10. The method of claim 1, wherein the one or more functional groups comprise amines (—

NH₂), imines (═

NH), hydroxyls (—

OH), esters (—

COOC—

), and carboxylic acids (—

COOH), halides, sulfides or combinations thereof.

11. A method for creating surface-tailored vinyl acetic polymer coating for improved cells proliferation comprising the steps of:

converting one or more vinylacetic acid monomers comprising one or more —

COOH groups to a monomer vapor;

contacting the monomer vapor and a substrate; and

igniting a radio frequency plasma glow discharge to polymerize the monomer vapor to form surface-tailored vinyl acetic polymer on the substrate at a thicknesses of between 25 and 300 nm and a —

COOH group surface density of between 3.6 and 9%, wherein the surface-tailored vinylacetic acid polymer enhances cell proliferation.

12. A surface-tailored polymer coated substrate with improved cells proliferation characteristics comprising:

a substrate have a 25 and 300 nm vapor deposited polymer coating comprising one or more functional groups with a functional group surface density of between 3 and 9%, wherein the 25 and 300 nm vapor deposited polymer coating enhances cell proliferation.

13. The surface-tailored polymer coated substrate of claim 12, wherein the one or more functional groups comprise amines (—

NH₂), imines (═

NH), hydroxyls (—

OH), esters (—

COOC—

), and carboxylic acids (—

COOH), halides, sulfides or combinations thereof.

14. The surface-tailored polymer coated substrate of claim 12, wherein the thicknesses is between 25 and 200 nm.

15. The surface-tailored polymer coated substrate of claim 12, wherein the 25 and 300 nm vapor deposited polymer coating comprises one or more ethylene acetic acid monomers, one or more vinylacetic acid monomers or a mixture thereof.

16. A method improving cell proliferation on a substrate comprising the steps of:

providing a substrate;

contacting the substrate with a monomer vapor comprising one or more monomers having one or more functional groups; and

igniting a radio frequency plasma glow discharge to polymerize the monomer vapor to form surface-tailored vinyl acetic polymer on the substrate at a thicknesses of between 25 and 300 nm and a functional group surface density of between 3.6 and 9%, wherein the surface-tailored polymer enhances cell proliferation.

17. The method of claim 16, wherein the one or more functional groups comprise amines (—

NH2), imines (═

NH), hydroxyls (—

OH), esters (—

COOC—

), and carboxylic acids (—

COOH), halides, sulfides or combinations thereof.

18. The method of claim 16, wherein the one or more monomers comprise one or more vinylacetic acid monomers, one or more acetic acid monomers, one or more ethylene acetic acid monomers or a combination thereof.

19. The method of claim 16, wherein the radio frequency plasma glow discharge is a continuous-wave radio frequency plasma glow discharge.

20. The method of claim 16, wherein the radio frequency plasma glow discharge is a pulsed-wave radio frequency plasma glow discharge.