DETERMINATION METHOD FOR ALLERGIC DISEASE
First Claim
1. A method for determining allergic diseases comprising activating a chemically-modified diamond/DLC (Diamond-like Carbon) chip with an activating reagent;
- conducting a coupling reaction with a peptide comprising allergen or allergen epitope;
subsequently contacting a sample with the allergen determination chip to which washing and blocking operations of unreacted active groups have been performed; and
detecting an allergen recognizing antibody in the sample captured by the allergen determination chip by an immunoassay using a labeled secondary antibody;
wherein a glycine-containing solution is used as a washing solution and/or blocking solution used in the washing and blocking operations.
2 Assignments
0 Petitions
Accused Products
Abstract
It is to provide a method for determining allergic diseases with a high sensitivity and accuracy which enables multilateral and global analysis with a minute amount of sample, even using body fluid other than blood such as saliva, nasal discharge and tears as a sample, particularly by suppressing nonspecific reaction as much as possible. A chemically modified diamond/DLC (Diamond-like Carbon) chip is activated with a reacting reagent, a coupling reaction with a peptide comprising allergen or allergen epitope is conducted, and a sample such as saliva, tears, and nasal discharge which has undergone pressure filtration with a low protein-adsorbing filter, is contacted with an allergen determination chip to which washing and blocking operations have been performed on unreacted active groups, and an allergen recognizing antibody in the sample captured by the allergen determination chip is detected by immunoassay using a labeled secondary antibody, wherein a glycine-containing solution is used for a washing solution and/or blocking solution used in the washing and blocking operations, is used.
17 Citations
15 Claims
-
1. A method for determining allergic diseases comprising activating a chemically-modified diamond/DLC (Diamond-like Carbon) chip with an activating reagent;
- conducting a coupling reaction with a peptide comprising allergen or allergen epitope;
subsequently contacting a sample with the allergen determination chip to which washing and blocking operations of unreacted active groups have been performed; and
detecting an allergen recognizing antibody in the sample captured by the allergen determination chip by an immunoassay using a labeled secondary antibody;
wherein a glycine-containing solution is used as a washing solution and/or blocking solution used in the washing and blocking operations. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- conducting a coupling reaction with a peptide comprising allergen or allergen epitope;
- 10. An allergy determination chip wherein a chemically modified diamond/DLC (Diamond-like Carbon) chip has been activated with an activating reagent, a coupling reaction has been conducted with a peptide comprising an allergen or allergen epitope, and washing and blocking operations of unreacted activated groups have been conducted with a washing solution and/or blocking solution containing glycine.
Specification