COMPOSITIONS AND METHODS FOR IMPROVED SACCHARIFICATION OF BIOMASS
First Claim
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1. A process for producing a fermentive end-product comprising:
- (a) contacting a carbonaceous biomass with;
i. a microorganism that hydrolyses and ferments said biomass; and
ii. an external source of one or more enzymes that are capable of enhancing said hydrolysis, and(b) allowing sufficient time for said hydrolysis and fermentation to produce a fermentive end-product;
wherein said one or more enzymes do not include a xylanase, a hemicellulase, a glucanase or glucosidase, and wherein said external source is not said microorganism.
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Abstract
Compositions and methods are provided for enhancing saccharification of biomass with one or more enzymes to enhance availability of substrates for fermentation by a microorganism. Microorganisms are also modified to enhance activity of one or more hydrolytic enzymes that are present endogenously in or are introduced heterologously into a host microorganism.
159 Citations
26 Claims
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1. A process for producing a fermentive end-product comprising:
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(a) contacting a carbonaceous biomass with; i. a microorganism that hydrolyses and ferments said biomass; and ii. an external source of one or more enzymes that are capable of enhancing said hydrolysis, and (b) allowing sufficient time for said hydrolysis and fermentation to produce a fermentive end-product; wherein said one or more enzymes do not include a xylanase, a hemicellulase, a glucanase or glucosidase, and wherein said external source is not said microorganism. - View Dependent Claims (4, 5, 6, 7, 8, 9, 11, 15, 17, 22, 23, 25)
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2. A process for producing a fermentive end-product comprising:
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(a) contacting a carbonaceous biomass with; i. a microorganism that hydrolyses and ferments said biomass; and ii. an external source of one or more enzymes that are capable of enhancing said hydrolysis, and (b) allowing sufficient time for said hydrolysis and fermentation to produce a fermentive end-product; wherein said one or more enzymes comprise a cellulase, and wherein the cellulase and the microorganism act synergistically to enhance hydrolysis. - View Dependent Claims (21)
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3. A process for producing a fermentive end-product comprising:
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(a) contacting a carbonaceous biomass with; i. a recombinant microorganism that hydrolyses and ferments said biomass; and ii. an external source of one or more enzymes that are capable of enhancing said hydrolysis, and (b) allowing sufficient time for said hydrolysis and fermentation to produce a fermentive end-product. - View Dependent Claims (10, 18, 19, 26)
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12. (canceled)
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16. (canceled)
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20. (canceled)
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24. (canceled)
Specification