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Methods, Surface Modified Plates and Compositions for Cell Attachment, Cultivation and Detachment

  • US 20100087002A1
  • Filed: 10/29/2009
  • Published: 04/08/2010
  • Est. Priority Date: 02/21/2008
  • Status: Abandoned Application
First Claim
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1. A method to enhance the attachment of cells to a surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer, comprising the steps of:

  • a. Obtaining a suspension of the cells,b. Treating the suspension of cells with at least one compound selected from the group consisting of;

    a compound capable of inhibiting Rho kinase activity, and a compound capable of inhibiting Rho activity, andc. Adding the suspension of cells to the surface and allowing the cells to attach.

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