Sample Collector and Processor

• US 20100087012A1
• Filed: 04/23/2008
• Published: 04/08/2010
• Est. Priority Date: 04/23/2007
• Status: Active Application

• Current Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Original Assignee: Advanced Liquid Logic, Inc. (Illumina Incorporated)
• Inventors: Shenderov, Alexander
• Application Number:
  US12/596,902
• Publication Number:
  US 20100087012A1
• Time in Patent Office: Days
• US Class Current: 436/518
• CPC Class Codes: G01N 1/2202 involving separation of sample components during sampling
• CPC Class Codes: G01N 2001/2217 using a liquid
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

An aerosol sample collector with an air flow path comprising: (i) at a first segment thereof, a particle charging device, and (i) at a second segment thereof, deflection plates configured to focus particles of a desired charge into a preselected cross-section of the air flow path. The air flow path also includes charged substrates arranged at an outflow portion of the air flow path to collect charged particles on a collection surface of the charged substrates; and an exit path for flowing particles not in the preselected cross-section of the air flow path away from the charged substrates. Related methods are also provided.

Claims

1. An aerosol sample collector comprising:

(a) an air flow path comprising;

(i) at a first segment thereof, a particle charging device; and

(ii) at a second segment thereof, deflection plates configured to focus particles of a desired charge into a preselected cross-section of the air flow path;

(b) charged substrates arranged at an outflow portion of the air flow path to collect charged particles on a collection surface of the charged substrates;

(c) an exit path for flowing particles not in the preselected cross-section of the air flow path away from the charged substrates.

2. The aerosol sample collector of claim 1 wherein the collection surface is hydrophobic in character.

3. The aerosol sample collector of claim 1 wherein the exit path is created by the use of louvers.

4. The aerosol sample collector of claim 1 wherein the collection surface and/or a surface separated from the collection surface by a gap comprises a droplet operations surface of a droplet actuator.

5. A system comprising:

(a) the aerosol sample collector of claim 4;

(b) a computer processor programmed to control droplet operations on the droplet actuator and further programmed to transport a droplet across the droplet operations surface to collect particles from the collection surface.

6. The system of claim 5:

(a) comprising one or more reagent droplets on the droplet operations surface; and

(b) further programmed to conduct droplet operations to execute an assay protocol using the sample droplet.

7. A system comprising:

(a) the aerosol sample collector of claim 1; and

(b) a robotic means for moving a droplet across the sample collection surface to collect particles from the collection surface, yielding a sample droplet comprising one or more particles.

8. An apparatus comprising:

(a) a substrate comprising a charged collection surface for collecting particles, wherein the charged collection surface comprises droplet operations electrodes associated therewith and serves as a droplet operations surface for droplet operations conducted by the electrodes; and

(b) a means for flowing particle-containing gaseous source material into contact with the collection surface.

9. A method of collecting particles, the method comprising:

(a) flowing air along a gaseous source material comprising the particles along a flow path;

(b) charging particles in the gaseous source material;

(c) using deflection plates appropriately positioned to focus the particles into a predetermined cross-section of the flow path; and

(d) collecting the particles onto a charged substrate while flowing gaseous source material not in the predetermined cross-section away from the charged substrate.

10. The method of claim 9 wherein the collection surface is hydrophobic in character.

11. The method of claim 9 wherein flowing gaseous source material not in the predetermined cross-section away from the charged substrate is accomplished a means comprising the use of louvers.

12. The method of claim 9 further comprising transporting a droplet across the surface of the collection substrate in order to collect particles into the droplet, thereby yielding a sample droplet comprising one or more of the particles.

13. The method of claim 12 further comprising transporting the droplet to a droplet operations surface of a droplet actuator for execution of one or more steps of a droplet based assay protocol using the droplet.

14. The method of claim 12 wherein the droplet comprises one or more beads having affinity for a target particle and/or affinity for a substance associated with the target particle.

15. The method of claim 14 further comprising transporting the droplet to a droplet operations surface of a droplet actuator for execution of one or more steps of a droplet based assay protocol using the droplet.

16. The method of claim 14 wherein the one or more steps comprise steps of a bead washing protocol.

17. The method of claim 9 wherein the transporting is mediated by a droplet actuator.

18. The method of claim 9 wherein the transporting is mediated by a robotic means.

19. The method of claim 17 wherein:

(a) the collection surface is also at least a portion of a droplet operations surface of a droplet actuator; and

/or

(b) the collection surface is also at least a portion of a top substrate of a droplet actuator and is separated by a gap from a droplet operations surface of a droplet actuator.

20. The method of claim 17 further comprising executing droplet operations to effect one or more steps of an assay protocol on the droplet operations surface using the sample droplet.

21. The method of claim 17 further comprising providing a reagent droplet on the collection surface and executing droplet operations to effect one or more steps of an assay protocol on the collection surface using the sample droplet and the at least one reagent droplet.

22. The method of claim 21 wherein one or more of the droplet operations is electrode mediated.

23. The method of claim 21 wherein one or more of the droplet operations is electrowetting mediated.

24. The method of claim 21 wherein one or more of the droplet operations is dielectrophoresis-mediated.

25. The method of claim 19 further comprising providing at least one reagent droplet on the droplet operations surface and executing droplet operations to effect one or more steps of an assay protocol on the droplet operations surface using the sample droplet and the at least one reagent droplet.

26. The method of claim 9 wherein the transporting is controlled by a computer.