FUNCTIONAL NUCLEIC ACIDS AND METHODS
First Claim
1. An expression vector comprising:
- a chimeric gene encoding a gene product comprising a selective nucleic acid within a carrier nucleic acid, wherein said selective nucleic acid is capable of binding to or affecting at least one target molecule;
wherein said expression vector when transfected into a host transcribes said chimeric gene.
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Abstract
The present invention relates to methods of generating amounts of selective nucleic acids. The present invention further relates to selective nucleic acids incorporated within non-coding nucleic acids, capable of binding to or altering a target molecule. Selective nucleic acids may generally refer to, but are not limited to, deoxyribonucleic acids (DNAs), ribonucleic acids (RNAs), artificially modified nucleic acids, combinations or modifications thereof. Selective nucleic acids may also generally refer to, but are not limited to, nucleic acid aptamers, aptazymes, ribozymes, deoxyribozymes, nucleic acid probes, small interfering RNAs (siRNAs), micro RNAs (miRNAs), short hairpin RNAs (shRNAs), antisense nucleic acids, diagnostic probes or probe libraries, aptamer inhibitors, precursors of any of the above and/or combinations or modifications thereof. In one aspect, a method for generating amounts of selective nucleic acids includes incorporating a selective nucleic acid sequence into a carrier nucleic acid. In general, the carrier nucleic acid may be transcribed by a cell into a product nucleic acid which may carry an incorporated selective nucleic acid sequence.
26 Citations
25 Claims
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1. An expression vector comprising:
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a chimeric gene encoding a gene product comprising a selective nucleic acid within a carrier nucleic acid, wherein said selective nucleic acid is capable of binding to or affecting at least one target molecule; wherein said expression vector when transfected into a host transcribes said chimeric gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. An isolated cell comprising:
at least one selective nucleic acid sequence incorporated into a genomic DNA encoding a non-coding nucleic acid, wherein said selective nucleic acid binds to or affects a target molecule. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
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20. A method for generating amounts of selective nucleic acids comprising:
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generating a library of nucleic acid sequences encoding selective nucleic acid capable of binding to or affecting at least one target molecule; incorporating said nucleic acid sequences in at least one carrier nucleic acid in a cell; culturing said cell to achieve a cell population; and purifying selective nucleic acids incorporated into carrier nucleic acids from said cell population. - View Dependent Claims (21, 22, 23, 24, 25)
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Specification