NON-ENZYMATIC ELECTROCHEMICAL METHOD FOR SIMULTANEOUS DETERMINATION OF TOTAL HEMOGLOBIN AND GLYCATED HEMOGLOBIN
First Claim
1. A screen printed electrode (SPE) strip for simultaneous measurement of total hemoglobin and percentage of glycated hemoglobin in a blood sample comprising four electrodes comprising a counter electrode, working electrode, reference electrode and an electrode modified by a water—
- insoluble boronic acid compound;
wherein the counter, working and reference electrodes are used for estimation of Hb by amperometry or differential pulse voltammetry;
the reference and modified electrodes are used for estimation of HbA1c by potentiometry; and
the reference electrode is used for both amperometry and potentiometry.
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Abstract
A non-enzymatic electrochemical method of simultaneous measurement of hemoglobin (Hb) and percentage of glycated hemoglobin (% HbA1c) in a blood sample is disclosed. The method includes determining the total amount of hemoglobin in a sample by electrochemically measuring the voltammetric current due to iron (II) and iron (III) redox portions in hemoglobin and determining the percentage of glycated hemoglobin (HbA1c) by potentiometry. Also disclosed is a novel screen-printed electrode (SPE) strip modified for potentiometric measurement of HbA1c.
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Citations
20 Claims
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1. A screen printed electrode (SPE) strip for simultaneous measurement of total hemoglobin and percentage of glycated hemoglobin in a blood sample comprising four electrodes comprising a counter electrode, working electrode, reference electrode and an electrode modified by a water—
- insoluble boronic acid compound;
wherein the counter, working and reference electrodes are used for estimation of Hb by amperometry or differential pulse voltammetry;
the reference and modified electrodes are used for estimation of HbA1c by potentiometry; andthe reference electrode is used for both amperometry and potentiometry. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
- insoluble boronic acid compound;
Specification