GOLD IMPLANTATION/DEPOSITION OF BIOLOGICAL SAMPLES FOR LASER DESORPTION TWO AND THREE DIMENSIONAL DEPTH PROFILING OF BIOLOGICAL TISSUES

• US 20100090101A1
• Filed: 12/04/2009
• Published: 04/15/2010
• Est. Priority Date: 06/04/2004
• Status: Abandoned Application

• Current Assignee: Ionwerks, Inc.
• Original Assignee: Ionwerks, Inc.
• Inventors: Tempez, Agnes, Ugarov, Michael V., Della-Negra, Serge, Le Bayec, Yvon, Schultz, J. Albert, Egan, Thomas F.
• Application Number:
  US12/630,922
• Publication Number:
  US 20100090101A1
• Time in Patent Office: Days
• US Class Current: 250/282
• CPC Class Codes: H01J 49/0418 for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
• CPC Class Codes: H01J 49/0481 with means for collisional cooling
• CPC Class Codes: H01J 49/142 using a solid target which is not previously vapourised

Abstract

The present invention enhances the laser desorption of biological molecular ions from surfaces by creating a surface localized MALDI particle matrix by ion implantation of low energy ionized clusters (gold, aluminum, etc.) or chemically derivatized clusters into the near surface region of the sample. MALDI analysis of the intact biomolecules on the surface or within a narrow subsurface region defined by the implantation range of the ions can then be performed by laser desorption into a mass spectrometer or, in a preferred embodiment, into a combined ion mobility orthogonal time of flight mass spectrometer.

Claims

1-33. -33. (canceled)

34. A method for creating and analyzing secondary ions from a sample comprising the steps of:

impinging incident ions onto said sample to form secondary ions;

cooling said secondary ions, said step of cooling comprising contacting said secondary ions with one or more gases; and

,

transporting said secondary ions into a mass spectrometer.