GENES USEFUL FOR DIAGNOSING AND MONITORING INFLAMMATION RELATED DISORDERS

• US 20100092989A1
• Filed: 12/10/2009
• Published: 04/15/2010
• Est. Priority Date: 09/08/2004
• Status: Abandoned Application

• Current Assignee: XDx Incorporated
• Original Assignee: XDx Incorporated
• Inventors: Walther, Dirk, WOHLGEMUTH, Jay, Lal, Preeti G., Tayama, Darren
• Application Number:
  US12/635,438
• Publication Number:
  US 20100092989A1
• Time in Patent Office: Days
• US Class Current: 435/6.14
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

Described herein is a system for monitoring gene expression for diagnosing and monitoring inflammation disorders, and for monitoring gene expression in inflammation disorders in response to a particular drug treatment regimen. This system for detecting nucleic acid expression in a body fluid uses an isolated polynucleotide to detect expression of a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO: 5; SEQ ID NO:11; SEQ ID NO:17; and SEQ ID NO: 23. These nucleic acids are differentially expressed in body fluid in an individual with a disease criterion for a disease as listed in Table 1 as compared to an individual without the disease criterion.

Claims

1. A method for determining the likelihood of a human patient to reject a cardiac transplant comprising detecting the level of a RNA that encodes SEQ ID NO:

12 in a blood sample from said patient and determining that said patient is likely to reject said cardiac transplant when the level of said RNA that encodes SEQ ID NO;

12 is increased relative to the mean level of said RNA that encodes SEQ ID NO;

12 in quiescent samples.

2. The method of claim 1 further comprising isolating RNA from said patient prior to detecting.

3. The method of claim 2 wherein said RNA is detected by PCR.

4. The method of claim 2 wherein said RNA is detected by hybridization.

5. The method of claim 2 wherein said RNA is detected by hybridization to an oligonucleotide.

6. The method of claim 5 wherein said oligonucleotide comprises DNA, RNA, cDNA, PNA, genomic DNA, or synthetic oligonucleotides.