USE OF LONGITUDINALLY DISPLACED NANOSCALE ELECTRODES FOR VOLTAGE SENSING OF BIOMOLECULES AND OTHER ANALYTES IN FLUIDIC CHANNELS
First Claim
1. A device for voltage sensing of analytes, the device comprising:
- a fluidic channel defined in a substrate;
a pair of sensing electrodes disposed in the channel for sensing voltage therein, the pair of sensing electrodes comprising a first and a second sensing electrode disposed at two discrete locations along a length of the fluidic channel; and
a pair of electromotive electrodes disposed at a first end and a second end of the fluidic channel for applying a potential along the fluidic channel,wherein the fluidic channel comprises a nanochannel or a microchannel.
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Accused Products
Abstract
Devices and methods for detecting an analyte are provided. Devices for voltage sensing of analytes may comprise a fluidic channel defined in a substrate, a pair of sensing electrodes disposed in a fluidic channel for sensing voltage therein, and a pair of electromotive electrodes for applying potential along the fluidic channel. The pair of sensing electrodes may include a first and second sensing electrode disposed at two discrete locations along the length of the fluidic channel and the pair of electromotive electrodes may be disposed at a first end and a second end of the fluidic channel. The fluidic channel may include a nanochannel or a microchannel. Methods for detecting an analyte may include the steps of disposing the analyte in a fluidic channel; applying a potential along the fluidic channel to generate an electrophoretic force therein such that the analyte is translocated from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the sensing electrodes.
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Citations
50 Claims
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1. A device for voltage sensing of analytes, the device comprising:
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a fluidic channel defined in a substrate; a pair of sensing electrodes disposed in the channel for sensing voltage therein, the pair of sensing electrodes comprising a first and a second sensing electrode disposed at two discrete locations along a length of the fluidic channel; and a pair of electromotive electrodes disposed at a first end and a second end of the fluidic channel for applying a potential along the fluidic channel, wherein the fluidic channel comprises a nanochannel or a microchannel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for detecting an analyte, the method comprising the steps of:
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disposing the analyte in a fluidic channel; applying a potential along the fluidic channel; translocating the analyte from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the pair of sensing electrodes, the pair of sensing electrodes comprising a first and a second electrode disposed at two discrete locations along a length of the fluidic channel, wherein the fluidic channel comprises a nanochannel or a microchannel. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method for determining a sequence of a biopolymer, the method comprising:
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preparing an analyte comprising the biopolymer; disposing the analyte in a fluidic channel; applying a potential along the fluidic channel; translocating the analyte from a first end of the fluidic channel to a second end of the fluidic channel; and measuring a voltage signal between a pair of sensing electrodes disposed in the fluidic channel as the analyte moves past the pair of sensing electrodes, the voltage signal corresponding to locations along the biopolymer, the pair of sensing electrodes comprising a first and a second electrode disposed at two discrete locations along a length of the fluidic channel, wherein the fluidic channel comprises a nanochannel or a microchannel. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification