CHROMATOGRAPHY QUANTITATIVE MEASURING APPARATUS

• US 20100098587A1
• Filed: 12/18/2009
• Published: 04/22/2010
• Est. Priority Date: 09/25/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Aga, Masahiro, Miyoshi, Koji, Shigematsu, Kaoru
• Granted Patent: US 8,722,424 B2
• Time in Patent Office: Days
• US Class Current: 422/70
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 30/95 Detectors specially adapted therefor; Signal analysis
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: Y10S 435/967 Standards, controls, materials, e.g. validation studies, buffer systems
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

In a chromatography quantitative measuring apparatus according to the present invention, a beam applied from a light source to a chromatography test strip is formed into an elliptical shape by an optical means such as a cylindrical lens, a variation in absorbance that accompanies elution of a marker regent is detected while the elliptical beam is applied between a marker reagent hold part and a detection part, and a measurement is automatically started in a prescribed period of time since the detection of variation. According to the chromatography quantitative measuring apparatus so configured, non-uniform coloration is reduced by shaping the beam elliptically with the optical means, whereby the accuracy of quantitative analysis is enhanced, and the apparatus can be operated easily.

Claims

1-14. -14. (canceled)

15. A chromatography quantitative measuring apparatus which applies an optical beam emitted from a light source to a chromatography test strip that comprises an application part where an inspection target solution is applied;

a marker reagent hold part which holds a marker reagent that can be eluted by development of the inspection target solution;

a base part where a specific binding reaction is caused between the marker reagent and an analysis target included in the inspection target solution; and

a detection where a bounded material of the marker reagent and the analysis target is immobilized, detects an optical signal utilizing a transmitted light or reflected light from the chromatography test strip, and quantitatively measures the concentration of the analysis target included in the inspection target solution from the signal, wherein

the inspection target solution is applied to the chromatography test strip, the optical beam is applied to a prescribed position of the base part, a variation of the transmitted light or reflected light from the chromatography test strip, which is generated by the elution of the marker reagent that accompanies the development of the inspection target solution, is detected, and the concentration of the analysis target included in the inspection target solution is measured in a prescribed period of time since the detection of variation.

16. The chromatography quantitative measuring apparatus as defined in claim 15, wherein

at least one of temperature and humidity is monitored, and a previously set prescribed period of time after which the measurement of the concentration of the analysis target is performed is compensated.

17. The chromatography quantitative measuring apparatus as defined in claim 15, wherein

the light source is repeatedly lighted and extinguished alternately while the development of the inspection target solution is detected.

18. The chromatography quantitative measuring apparatus as defined in claim 15, wherein

the light source is extinguished until shortly before the detection of the development of the inspection target solution.

19. The chromatography quantitative measuring apparatus as defined in claim 15, wherein

output from the light source is set lower than that when the concentration of the analysis target is measured, while the development of the inspection target solution is detected.

20-88. -88. (canceled)