COMPOSITIONS, METHODS, AND KITS FOR POLYUNSATURATED FATTY ACIDS FROM MICROALGAE
First Claim
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1. A process of making a polyunsaturated fatty acid composition comprising at least 8% polyunsaturated fatty acids in weight, the process comprising:
- extracting the polyunsaturated fatty acids from a microalgae, wherein,(a) SDA is in an amount of about 5% to about 50% of total fatty acids;
(b) EPA is in an amount of about 2% to about 30% of total fatty acids;
(c) DHA is in an amount of about 2% to about 30% of total fatty acids; and
optionally(d) GLA is in an amount of about 0.1% to about 10% of total fatty acids;
wherein the composition comprises at least 8% polyunsaturated fatty acids in weight.
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Abstract
The present invention provides compositions, methods, and kits comprising PUFAs produced by microalgae, in particular omega-3 and/or omega-6 fatty acids produced by members of the genus Rhodomonas, in particular Rhodomonas salina. The invention also provides compositions, methods, and kits comprising the PUFAs for the prophylactic and/or therapeutic treatment of a disease or condition, in particular a cardiovascular and/or inflammatory disease or condition.
103 Citations
80 Claims
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1. A process of making a polyunsaturated fatty acid composition comprising at least 8% polyunsaturated fatty acids in weight, the process comprising:
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extracting the polyunsaturated fatty acids from a microalgae, wherein, (a) SDA is in an amount of about 5% to about 50% of total fatty acids; (b) EPA is in an amount of about 2% to about 30% of total fatty acids; (c) DHA is in an amount of about 2% to about 30% of total fatty acids; and
optionally(d) GLA is in an amount of about 0.1% to about 10% of total fatty acids; wherein the composition comprises at least 8% polyunsaturated fatty acids in weight. - View Dependent Claims (2, 3, 4, 5, 16, 17, 20, 22)
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6. A process of making a composition comprising at least 5% stearidonic acid, the process comprising:
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(a) cultivating a microalgae to produce a microalgae biomass; and
either(b) extracting said microalgae oil from said cultivated microalgae;
or(c) removing water from said microalgae biomass to achieve a solids content from 5 to 100% weight percent; wherein the composition comprises at least 5% stearidonic acid. - View Dependent Claims (18, 19, 21, 23)
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7. A process of making an animal feed additive comprising fatty acids from a microalgae, the process comprising:
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(a) cultivating microalgae to produce a microalgae biomass; and
either(b) extracting microalgae oil from said microalgae biomass to produce a microalgae oil;
or(c) removing water from said microalgae biomass to produce a microalgae biomass with a solids content from 5% to 100% weight percent; wherein the animal feed additive comprises fatty acids from a microalgae. - View Dependent Claims (8, 24, 26, 28)
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9. A process of making an animal feed additive comprising at least 8% polyunsaturated fatty acids in weight;
- the process comprising;
extracting the fatty acids from a microalgae, wherein, (a) SDA is in an amount of about 5% to about 50% of total fatty acids; (b) EPA is in an amount of about 2% to about 30% of total fatty acids; (c) DHA is in an amount of about 2% to about 30% of total fatty acids; and
optionally(d) GLA is in an amount of about 0.1% to about 10% of total fatty acids; wherein the animal feed additive comprises at least 8% polyunsaturated fatty acids. - View Dependent Claims (25, 27, 29)
- the process comprising;
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10. A process of producing an animal having an increased tissue content of long chain omega-3 fatty acids, the method comprising feeding to an animal an animal feed additive comprising microalgae polyunsaturated fatty acids, the microalgae polyunsaturated fatty acids comprising:
- (a) a microalgae oil extracted from a cultivated microalgae biomass and/or (b) a microalgae biomass from a cultivated microalgae, wherein water is removed from microalgae biomass to achieve a solids content from 5 to 100%;
wherein an animal is produced having increased tissue content of long chain omega-3 fatty acids. - View Dependent Claims (12, 30, 32)
- (a) a microalgae oil extracted from a cultivated microalgae biomass and/or (b) a microalgae biomass from a cultivated microalgae, wherein water is removed from microalgae biomass to achieve a solids content from 5 to 100%;
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11. A process of producing an animal having an increased tissue content of long chain omega-3 fatty acids, the process comprising feeding to an animal an animal feed additive comprising at least 8% polyunsaturated fatty acids in weight;
- the animal feed additive comprising polyunsaturated fatty acids extracted from a microalgae, wherein (a) SDA is in an amount of about 5% to about 50% of total fatty acids;
(b) EPA is in an amount of about 2% to about 30% of total fatty acids;
(c) DHA is in an amount of about 2% to about 30% of total fatty acids; and
optionally (d) GLA is in an amount of about 0.1% to about 10% of total fatty acids;
wherein an animal is produced having an increased tissue content of long chain omega-3 fatty acids. - View Dependent Claims (13, 31, 33)
- the animal feed additive comprising polyunsaturated fatty acids extracted from a microalgae, wherein (a) SDA is in an amount of about 5% to about 50% of total fatty acids;
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14. A method of treating a mammalian disease in a subject in need thereof by administration to the subject a therapeutically effective amount of a polyunsaturated fatty acid composition comprising at least 8% polyunsaturated fatty acids in weight extracted from a microalgae, wherein the microalgae fatty acid extract comprises,
(a) SDA in an amount of about 5% to about 50% of total fatty acids; -
(b) EPA in an amount of about 2% to about 30% of total fatty acids; (c) DHA in an amount of about 2% to about 30% of total fatty acids; and
optionally(d) GLA in an amount of about 0.1% to about 10% of total fatty acids.
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15. A method of treating a mammalian disease in a subject in need thereof by administration to the subject a therapeutically effective amount of a composition comprising at least 5% SDA in a non-phospholipid form, the composition comprising either (a) a microalgae oil extracted from a cultivated microalgae biomass or (b) a microalgae biomass from a cultivated microalgae, wherein water is removed from microalgae biomass to achieve a solids content from about 5 to 100% weight percent.
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34. A method for preparing a microalgae biomass comprising one or more PUFAs, the method comprising:
culturing a microalgae under a culture condition sufficient to provide a microalgae biomass comprising the one or more PUFAs, wherein the microalgae biomass is harvested after at least one logarithmic growth phase. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 45, 46, 47, 48, 49, 55, 56, 57, 73, 74, 75, 76, 77)
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44. A method for preparing a microalgae biomass comprising one or more PUFAs, the method comprising:
culturing a microalgae under a culture condition sufficient to provide a microalgae biomass comprising the one or more PUFAs, wherein the microalgae biomass is harvested after at least one logarithmic growth phase of the microalgae, wherein the omega-3 fatty acid is stearidonic acid (SDA), wherein the omega-6 fatty acid is γ
-linolenic acid (GLA), wherein the SDA at harvest is at least about 30% of the total fatty acid, wherein the GLA at harvest is less than about 1% of total fatty acid.
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50. A lipid composition prepared from a microalgae biomass, wherein the lipid composition, upon extraction from the microalgae biomass, comprises:
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(a) a microalgae-produced SDA in a total SDA amount of at least 10% of the total fatty acids; and (b) a microalgae-produced GLA in a total GLA amount of less than about 5% of the total fatty acids. - View Dependent Claims (51)
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52. A microalgae biomass comprising lipids having at least 30% by weight of total fatty acids as omega-3 fatty acids.
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53. A microalgae biomass comprising lipids having at least 40% by weight of total fatty acids as omega-3 and omega-6 fatty acids.
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54. A microalgae biomass comprising an omega-3 fatty acid content of at least 10% dry weight of the biomass.
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58. A method for preparing a microalgae biomass comprising one or more PUFAs, the method comprising:
culturing a microalgae under a culture condition sufficient to provide a microalgae biomass comprising the one or more PUFAs, wherein the microalgae biomass is harvested at a negative growth acceleration phase or a stationary phase of the microalgae. - View Dependent Claims (59, 60, 61, 62, 63, 64, 65, 66, 67, 70, 71, 72)
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68. A lipid composition prepared from a microalgae biomass, wherein the lipid composition, upon extraction from the microalgae biomass, comprises:
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(a) a microalgae-produced SDA in a total SDA amount of at least 5% of the total fatty acids; and (b) a microalgae-produced GLA in a total GLA amount of at least 0.1% of the total fatty acids. - View Dependent Claims (69)
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- 78. An isolated microalgae of the Rhodomonas strain 12Rsp.
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80. A biologically pure culture of CCMP757.
Specification