METHOD FOR QUANTITATING SUBSTANCE TO BE MEASURED

• US 20100100335A1
• Filed: 10/31/2006
• Published: 04/22/2010
• Est. Priority Date: 10/31/2006
• Status: Active Grant

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: Miyagawa, Haruhiko
• Granted Patent: US 8,145,438 B2
• Time in Patent Office: Days
• US Class Current: 702/23
• CPC Class Codes: G01N 2030/045 internal
• CPC Class Codes: G01N 30/7206 interfaced to gas chromatograph interfaces in general for introducing or extracting samples to be analysed with specially adapted mass spectrometer, see H01J49/04
• CPC Class Codes: G01N 30/8679 Target compound analysis, i.e. whereby a limited number of peaks is analysed

Abstract

A method for quantitating a substance to be measured according to which a substance to be measured is quantitated is characterized by including: a database storing step of storing a database with which a relative calibration curve is registered; an internal standard substance measuring step of measuring an internal standard substance having a known concentration so that a mass spectrum of the internal standard substance is gained; an absolute value calculating step of calculating the peak area or the height of an internal standard substance by preparing a chromatogram on the basis of the mass spectrum of the internal standard substance; a substance to be measured measuring step of gaining a mass spectrum of the substance to be measured by measuring the substance to be measured; a measured value calculating step of calculating the peak area or the height of the substance to be measured by preparing a chromatogram on the basis of the mass spectrum of the substance to be measured; and a concentration of a substance to be measured calculating step of calculating the concentration of the substance to be measured on the basis of the relative calibration curve, the peak area or the height of the internal standard substance and the peak area or the height of the substance to be measured.

Claims

1. A method for quantitating a substance to be measured, according to which a substance to be measured is quantitated using a chromatographic mass spectrometer, characterized by comprising:

a database storing step of storing a database, with which a relative calibration curve is registered showing the relationship between a peak area ratio or a height ratio in the chromatogram of a standard substance to a registered substance and the concentration ratio of the standard substance to the registered substance;

an internal standard substance measuring step of measuring an internal standard substance having a known concentration so that a mass spectrum of the internal standard substance is gained;

an absolute value calculating step of calculating the peak area or the height of an internal standard substance by preparing a chromatogram on the basis of the mass spectrum of the internal standard substance;

a substance to be measured measuring step of gaining a mass spectrum of the substance to be measured by measuring the substance to be measured;

a measured value calculating step of calculating the peak area or the height of the substance to be measured by preparing a chromatogram on the basis of the mass spectrum of the substance to be measured; and

a concentration of a substance to be measured calculating step of calculating the concentration of the substance to be measured on the basis of the relative calibration curve, the peak area or the height of the internal standard substance and the peak area or the height of the substance to be measured.

2. The method for quantitating a substance to be measured according to claim 1, characterized in that an absolute calibration curve showing the relationship between the peak area or height and the concentration is prepared on the basis of the peak area or the height of the relative calibration curve and the internal standard substance in the concentration of the substance to be measured calculating step.

3. The method for quantitating a substance to be measured according to claim 1, characterized in that

said registered substance is a deuterium body where hydrogen atoms are substituted with deuterium atoms, and

the hydrogen atoms are not substituted with deuterium atoms in said internal standard substance.

4. The method for quantitating a substance to be measured according to claim 2, characterized in that

said registered substance is a deuterium body where hydrogen atoms are substituted with deuterium atoms, and

the hydrogen atoms are not substituted with deuterium atoms in said internal standard substance.