CHROMATOGRAPHY QUANTITATIVE MEASURING APPARATUS

• US 20100104475A1
• Filed: 12/18/2009
• Published: 04/29/2010
• Est. Priority Date: 09/25/2000
• Status: Active Grant

• Current Assignee: PHC Holdings Corporation
• Original Assignee: Panasonic Healthcare Holdings Co., Ltd.
• Inventors: Aga, Masahiro, Miyoshi, Koji, Shigematsu, Kaoru
• Granted Patent: US 8,722,425 B2
• Time in Patent Office: Days
• US Class Current: 422/70
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 30/95 Detectors specially adapted therefor; Signal analysis
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: Y10S 435/967 Standards, controls, materials, e.g. validation studies, buffer systems
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

In a chromatography quantitative measuring apparatus according to the present invention, a beam applied from a light source to a chromatography test strip is formed into an elliptical shape by an optical means such as a cylindrical lens, a variation in absorbance that accompanies elution of a marker regent is detected while the elliptical beam is applied between a marker reagent hold part and a detection part, and a measurement is automatically started in a prescribed period of time since the detection of variation. According to the chromatography quantitative measuring apparatus so configured, non-uniform coloration is reduced by shaping the beam elliptically with the optical means, whereby the accuracy of quantitative analysis is enhanced, and the apparatus can be operated easily.

Claims

1-23. -23. (canceled)

24. A chromatography quantitative measuring apparatus which applies an optical beam emitted from a light source to a chromatography test strip that comprises an application part where an inspection target solution is applied;

a marker reagent hold part which holds a marker reagent that can be eluted by development of the inspection target solution;

a base part where a specific binding reaction is caused between the marker reagent and an analysis target included in the inspection target solution; and

a detection part where a bounded material of the marker reagent and the analysis target is immobilized, detects an optical signal utilizing a transmitted light or reflected light from the chromatography test strip, and quantitatively measures the concentration of the analysis target included in the inspection target solution from the signal, wherein

a kind of inspection target solution is judged from a detection signal at the base part on the chromatography test strip where the inspection target solution is applied.

25. The chromatography quantitative measuring apparatus as defined in claim 24, wherein

the base part where the detection signal is measured is located downstream of the detection part in the direction of the development.

26. The chromatography quantitative measuring apparatus as defined in claim 24, wherein

a calibration curve in conformity with the inspection target solution can be selected previously.

27-88. -88. (canceled)