Use of advanced nanomaterials for increasing sepecific cell functions

• US 20100104652A1
• Filed: 10/23/2009
• Published: 04/29/2010
• Est. Priority Date: 10/27/2008
• Status: Abandoned Application

• Current Assignee: The Board of Trustees of the University of Arkansas (University of Arkansas System)
• Original Assignee: University of Kansas
• Inventors: Mahmood, Meena, Biris, Alexandru S., Jensen, Peder
• Application Number:
  US12/588,679
• Publication Number:
  US 20100104652A1
• Time in Patent Office: Days
• US Class Current: 424/490
• CPC Class Codes: A61K 9/5115 Inorganic compounds
• CPC Class Codes: B22F 1/054 Nanosized particles
• CPC Class Codes: B22F 1/16 Metallic particles coated with a non-metal coated with lubricating or binding agents or with organic material B22F1/10
• CPC Class Codes: B22F 1/18 Non-metallic particles coated with metal
• CPC Class Codes: B22F 2998/00 Supplementary information concerning processes or compositions relating to powder metallurgy
• CPC Class Codes: C22C 5/02 Alloys based on gold
• CPC Class Codes: C22C 5/06 Alloys based on silver

Abstract

Disclosed herein are methodologies and compositions for enhancing cellular functions, which can be used in a variety of biological applications.

Claims

1. A method for regulating a cellular function, comprising

(a) combining a material and a nanoparticle composition to form a material system, wherein said combining is by coating or binding;

and

(b) delivering said material system to a cell type or tissue.

2. The method of claim 1, wherein said material is for a biomedical application.

3. The method of claim 1, wherein said material is at a nano, micro, or macro scale.

4. The method of claim 1, wherein said nanoparticle composition comprises gold, metal, metal oxide, polymer, titanium dioxide, silver, carbon nanotubes, hydroxyapatite, quantum dots, crystals, salts, ceramic materials, magnetic materials, or any combination thereof.

5. The method of claim 1, wherein said nanoparticle composition comprises a plurality of materials.

6. The method of claim 5, wherein said plurality comprises

(a) a core made of one material;

and

(b) at least one layer surrounding said core, wherein said layer comprises a material that is not the same as said core.

7. The method of claim 1, wherein said nanoparticle composition comprises material having a size from about 0.5 nm to about 50 mm

8. The method of claim 6, wherein said material has a size from about 50 microns to 50 mm.

9. The method of claim 1, wherein said material is coated with at least one layer of coating that delivers a drug or bioactive agent, wherein said drug or bioactive agent prevents oxidation, prevents or treats infection, increases bio-compatibility, increases or decreases a cell function, promotes cell adhesion and proliferation, reduces toxicity, or promotes binding with a biological or non-biological system.

10. The method of claim 1, wherein said delivering is by injection, epidermal translation, inhalation, surgical delivery, topical application, oral administration or any other method that targets a desired cell type or tissue.

11. The method of claim 1, wherein said cellular function is at least one of bone formation, protein synthesis, gene expression, cell proliferation, mitosis, DNA transcription, hormone production, enzyme production, cell death, gene delivery, or drug delivery.

12. The method of claim 1, wherein said material is a natural or synthetic polymer, metal, metal oxide, metal nitride, borate, ceramic, allograft hard tissue, allograft soft tissue, xenograft hard tissue, xenograft soft tissue, carbon nanostructure, glasses, or natural, or biocompatible material.

13. The method of claim 1, wherein said material is coated with an oxide,nitride, borate, polymer, ceramic, zirconia, metal, metal oxide, carbon, or graphitic material.

14. The method of claim 1, wherein said material system is capable of preventing or treating an infection.

15. The method of claim 14, wherein said infection is a bacterial, fungal, prion, parasite, or viral infection.

16. The method of claim 12, wherein said carbon nanostructure is a single walled carbon nanotube, double walled carbon nanotube, multi walled carbon nanotube, nanofiber, fullerene, or grapheme.

17. The method of claim 1, wherein said tissue is bone tissue.

18. The method of claim 1, wherein said tissue is soft tissue.

19. A method for regulating a cellular function, comprising delivering nanoparticles in a solution, aerosol, gel, cream, paste to a cell type or tissue.

20. The method of claim 19, wherein said delivery is by injection, inhalation, oral administration, or topical application.

21. A device for regulating at least one cellular function, wherein said device comprises a material system.

22. The device of claim 21, wherein said device is an implant, graft, needle, catheter, dental implant, gel, cream, injectible, orthopedic implant, prosthetic, cardiovascular stent, defibrillator/pacemaker, medical tube, tissue engineering matrix or scaffold.

26. The device of claim 22, wherein said scaffold is a tissue-forming scaffold.

23. A device comprising nanoparticles, wherein said nanoparticles are layered throughout and/or on the surface of said device.

24. The device of claim 23, wherein said device is biodegradable and/or biocompatible.

25. The device of claim 23, wherein said nanoparticles are released from said device as each layer degrades.

27. A composition for promoting tissue growth, wherein said composition comprises nanoparticles.

28. The composition of claim 27, wherein said nanoparticles are layered in a surface coating covering.

29. The composition of claim 27, wherein said nanoparticles comprise gold, silver, metals, metal oxides, oxides, polymers, ceramics, carbon nanotubes, or hydroxyapatite.

30. A coating for an implant;

wherein said coating comprises nanoparticles.

31. The coating of claim 30, wherein said implant is polymeric, metal, metal alloy, shape memory metal, metal oxide, oxide, ceramic, zirconia, or hydroxyapatite.

32. A method for regulating a cellular function, comprising

(a) linking at least one nanoparticle to an agent to create a stimulating agent;

and

(b) delivering said stimulating agent to a cell type or tissue.

33. The method of claim 32, wherein said agent comprises a protein, growth factor, antibody, amino acids, polymers, drug(s), hormone, nucleic acid, peptide, or enzyme.

34. The method of claim 32, wherein said cellular function is bone growth and said stimulating agent is an osteoblast stimulating agent.

35. The method of claim 32, wherein said linking is covalent, polar covalent, ionic, sulfide, hydrogen bond, or any other linkage suitable for in vivo.

36. The method of claim 33, wherein said growth factor is Bone Morphogenic Proteins (BMPs), Brain-Derived Neutrophic Factor (BDNF), Ciliary Neutrophic Factor (CNTF), Epidermal Growth Factor (EGF), Erythropoietin (EPO), Fibroblast Growth Factor (FGF), Granulocyte-Colony Stimulating Factor (G-CSF), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Growth Differentiation Factor-9 (GDF9), Hepatocyte Growth Factor (HGF), Insulin-like Growth Factor (IGF), Interleukin (IL), Leukemia Inhibitory Factor (LIF), Myostatin (GDF-8), Nerve Growth Factor (NGF), Neutrophic Factors (NT), Platelet-derived Growth Factor (PDGF), Thrombopoietin (TPO), Transforming Growth Factor alpha(TGF-α

), Transforming Growth Factor beta (TGF-β

), or Vascular Endothelial Growth Factor (VEGF).

37. The method of claim 32, wherein said delivering is by injection, surgical placement, oral administration, inhalation, or transdermal application.

38. The method of claim 32, further comprising (c) exposing said cell or tissue comprising said stimulating agent to radiation for faster penetration.

39. The method of claim 38, wherein said radiation is laser radiation or electromagnetic radiation.

40. The method of claim 32, wherein said stimulating agent is placed in a solution, gel, paste, suspension, or aerosol, for delivery to said cell type or tissue.