QUANTITATIVE MEASUREMENT OF GLYCATED HEMOGLOBIN

• US 20100105020A1
• Filed: 10/23/2007
• Published: 04/29/2010
• Est. Priority Date: 10/24/2006
• Status: Abandoned Application

• Current Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Original Assignee: Koninklijke Philips Electronics N.V. (Koninklijke Philips N.V.)
• Inventors: Schmidt, Kristiane Anne, Lucassen, Gerhardus Wilhelmus
• Application Number:
  US12/444,435
• Publication Number:
  US 20100105020A1
• Time in Patent Office: Days
• US Class Current: 435/2
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G01N 33/723 Glycosylated haemoglobin

Abstract

The invention relates to a method for quantitative measurement of glycated hemoglobin (HbAIc) in a blood sample containing hemoglobin molecules. In this method, the hemoglobin molecules are extracted from the blood sample, adsorbed onto a roughened metal surface and subjected to a Surface Enhanced Raman Scattering (SERS) measurement. The invention provides a universal method for the measurement of the HbAIc concentration in blood.

Claims

1. - Method for quantitative measurement of glycated hemoglobin (HbA1c) in a blood sample containing hemoglobin molecules, the method comprising:

extracting the hemoglobin molecules from the blood sample,

adsorbing the hemoglobin molecules onto a roughened metal surface and

analyzing the spectrum obtained by a Surface Enhanced Raman Scattering of the adsorbed molecules for the quantitative measurement of glycated hemoglobin in the blood sample.

2. - Method according to claim 1, wherein the Raman scattering is further enhanced by means of Resonance Raman Scattering.

3. - Method according to claim 2, wherein the Raman scattering measurement being performed with a laser (2), the laser wavelength is chosen in a normal hemoglobin (HbA) absorption band.

4. - Method according to claim 1, wherein the concentration of normal hemoglobin (HbA) and the concentration of glycated hemoglobin (HbA1c) are determined simultaneously.

5. - Method according to claim 1, which comprises the following steps:

a) separation of blood cells from plasma in the blood sample (S1);

b) lysis of blood cells in order to release the hemoglobin molecules (S2);

c) addition of aggregated silver colloid to the hemoglobin molecules (S3);

d) Surface Enhanced Raman Scattering measurement of the hemoglobin molecules (S4);

e) analysis of the measured data in order to obtain a Raman spectrum (S5);

f) deduction of the glycated hemoglobin (HbA1c) concentration from the spectrum (S6).

6. - Method according to claim 5, comprising, between step a) and step b), a further step consisting in incubation of the cell fraction obtained in step a) with a saline solution to remove labile glycated hemoglobin with a Schiff base (pre-HbA1c).

7. - Device (1, 21) for quantitative measurement of glycated hemoglobin (HbA1c) in a blood sample containing hemoglobin molecules, comprising:

means (9, 10) for extracting the hemoglobin molecules from the blood sample,

means (11, 12) for adsorbing the hemoglobin molecules onto a roughened metal surface and

means ((2, 5, 7, 8), (2, 5, 13)) for analyzing the spectrum obtained by a Surface Enhanced Raman Scattering of the adsorbed molecules.

8. - Device according to claim 7, wherein the means (9, 10) for extracting the hemoglobin molecules from the blood sample comprise a first module (9) for the separation of blood cells from plasma and a second module (10) for the extraction of glycated and normal hemoglobin molecules from the blood cells.

9. - Device according to claim 7, wherein the means (11, 12) for adsorbing the hemoglobin molecules onto a roughened metal surface comprise a first source and means (11) for contacting the extracted molecules with an aggregating agent and a second source and means (12) for contacting the extracted molecules with silver colloidal particles.

10. - Device according to claim 7, wherein the means ((2, 5, 7, 8), (2, 5, 13)) for analyzing the spectrum obtained by a Surface Enhanced Raman Scattering of the adsorbed molecules comprise a measurement chamber (5), a light source (2) and a detection unit ((7, 8), 13) for the detection of Surface Enhanced Raman Scattering signals.

11. - Device according to claim 7, which is automated.