MICROFLUIDIC SYSTEMS INCLUDING POROUS POLYMER ELECTRODES
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Abstract
Microfluidic devices that incorporate a porous polymer electrode assemblies, including microfluidic device useful for detection of nucleic acids, as well as methods of using the microfluidic devices.
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Citations
30 Claims
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1-15. -15. (canceled)
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16. A microfluidic device for analyzing nucleic acids, comprising
a substrate having a plurality of microfluidic chambers and channels fabricated therein; -
a cover adhering to the substrate surface; an inlet configured to receive a biological sample; one or more chambers configured for pretreatment of the biological sample; one or more chambers configured for subjecting the biological sample to a polymerase chain reaction; one or more chambers configured to separate a nucleic acid polymer amplified by the polymerase chain reaction; and a porous polymer electrode configured to detect the amplified nucleic acid polymer.
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17. The microfluidic device of paragraph 16, further comprising a chamber configured to associate the amplified nucleic acid polymer with an electrochemically detectable label.
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18. The microfluidic device of paragraph 16, where the chambers configured to separate the amplified nucleic acid polymer are configured to separate the amplified nucleic acid polymer by electrophoresis
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19-25. -25. (canceled)
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26. A method of detecting a nucleic acid, comprising:
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introducing a sample thought to contain a target nucleic acid into the microfluidic device of any of paragraphs 16-19; pretreating the sample; subjecting the pretreated sample to the polymerase chain reaction; separating amplified nucleic acid polymers from the polymerase chain reaction mixture; and detecting the amplified nucleic acid polymers using the porous polymer electrode.
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27. The method of paragraph 23, where pretreating the sample includes one or more of digestion, liquidation, dilution, lysis, and denaturing the sample.
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28. The method of paragraph 26, further comprising labeling the amplified nucleic acid polymers with an electrochemically active label.
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29. The method of paragraph 28, further comprising labeling the amplified nucleic acid polymers with a specific complexing protein.
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30. The method of paragraph 26, where separating the amplified nucleic acid polymers includes electrophoretically separating the amplified nucleic acid polymers from the polymerase chain reaction mixture.
Specification