MICROFLUIDIC SYSTEMS INCLUDING POROUS POLYMER ELECTRODES

• US 20100105040A1
• Filed: 07/21/2009
• Published: 04/29/2010
• Est. Priority Date: 06/30/2005
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Inventors: Faulstich, Konrad, Lau, Aldrich N.K., Scaboo, Kristian M.
• Application Number:
  US12/507,045
• Publication Number:
  US 20100105040A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: G01N 27/44704 Details; Accessories

Abstract

Microfluidic devices that incorporate a porous polymer electrode assemblies, including microfluidic device useful for detection of nucleic acids, as well as methods of using the microfluidic devices.

Claims

1-15. -15. (canceled)

16. A microfluidic device for analyzing nucleic acids, comprising

a substrate having a plurality of microfluidic chambers and channels fabricated therein;

a cover adhering to the substrate surface;

an inlet configured to receive a biological sample;

one or more chambers configured for pretreatment of the biological sample;

one or more chambers configured for subjecting the biological sample to a polymerase chain reaction;

one or more chambers configured to separate a nucleic acid polymer amplified by the polymerase chain reaction; and

a porous polymer electrode configured to detect the amplified nucleic acid polymer.

17. The microfluidic device of paragraph 16, further comprising a chamber configured to associate the amplified nucleic acid polymer with an electrochemically detectable label.

18. The microfluidic device of paragraph 16, where the chambers configured to separate the amplified nucleic acid polymer are configured to separate the amplified nucleic acid polymer by electrophoresis

19-25. -25. (canceled)

26. A method of detecting a nucleic acid, comprising:

introducing a sample thought to contain a target nucleic acid into the microfluidic device of any of paragraphs 16-19;

pretreating the sample;

subjecting the pretreated sample to the polymerase chain reaction;

separating amplified nucleic acid polymers from the polymerase chain reaction mixture; and

detecting the amplified nucleic acid polymers using the porous polymer electrode.

27. The method of paragraph 23, where pretreating the sample includes one or more of digestion, liquidation, dilution, lysis, and denaturing the sample.

28. The method of paragraph 26, further comprising labeling the amplified nucleic acid polymers with an electrochemically active label.

29. The method of paragraph 28, further comprising labeling the amplified nucleic acid polymers with a specific complexing protein.

30. The method of paragraph 26, where separating the amplified nucleic acid polymers includes electrophoretically separating the amplified nucleic acid polymers from the polymerase chain reaction mixture.