Somatic Cells for Use in Cell Therapy
First Claim
1. A preserved cell preparation essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the preserved cell preparation comprising somatic cells and at least one polyphenol, wherein upon reconstitution of cells in the cell preparation, at least a portion of said stem cells are viable, said portion being sufficient for use of the cell preparation in stem cell therapy.
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Accused Products
Abstract
The present invention generally concerns cell therapy and products for use in such therapy. Particularly, the invention provides a preserved cell preparation essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the preserved cell preparation comprising somatic cells and at least one polyphenol, wherein upon reconstitution of cells in the cell preparation, at least a portion of said stem cells are viable, said portion being sufficient for use of the cell preparation in stem cell therapy. The invention also provides cells reconstituted from preserved somatic cells, and the use of the reconstituted cells in cell therapy. A preferred cell preparation in accordance with the invention comprises stem cells, preferably human stem cells.
55 Citations
41 Claims
- 1. A preserved cell preparation essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the preserved cell preparation comprising somatic cells and at least one polyphenol, wherein upon reconstitution of cells in the cell preparation, at least a portion of said stem cells are viable, said portion being sufficient for use of the cell preparation in stem cell therapy.
- 18. Use of a preserved cell preparation for the production of a therapeutic composition for cell therapy, the preserved cell preparation being essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the preserved cell preparation comprising somatic cells, wherein upon reconstitution of cells in the cell preparation, at least a portion of said cells are viable, said portion being sufficient for use of the therapeutic composition in cell therapy.
- 22. A reconstituted cell preparation being essentially free of one or more members of a group of cryoprotecting agents comprising polyalcohol, DMSO and cryoprotecting proteins, the reconstituted cell preparation comprising post-preservation somatic cells and at least one polyphenol, wherein at least a portion of said post-preservation somatic cells are viable, said portion being sufficient for use of the reconstituted cell preparation in cell therapy.
- 37. A method of treating a subject in need of somatic cell transplantation, the method comprises administering to said subject a cell preparation reconstituted from preservation conditions, the cell preparation being essentially free of one or more members of a group of cryoprotecting agents consisting of polyalcohols, DMSO and cryoprotecting proteins, the cell preparation comprising a effective amount of post-preservation and viable somatic cells the amount of the viable cells being sufficient for establishing said somatic cells in the subjects body.
Specification