SUPPORT HAVING PROTEIN IMMOBILIZED THEREON AND METHOD OF PRODUCING THE SAME

• US 20100105879A1
• Filed: 03/04/2008
• Published: 04/29/2010
• Est. Priority Date: 03/28/2007
• Status: Abandoned Application

• Current Assignee: JSR Corporation
• Original Assignee: JSR Corporation
• Inventors: Murata, Mitsuhiro, Katayose, Satoshi, Fukuta, Tetsuo
• Application Number:
  US12/529,824
• Publication Number:
  US 20100105879A1
• Time in Patent Office: Days
• US Class Current: 530/402
• CPC Class Codes: C07K 17/06 attached to the carrier via a bridging agent
• CPC Class Codes: G01N 33/543 with an insoluble carrier for immobilising immunochemicals
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393

Abstract

A method of producing a protein-immobilized support includes immobilizing a protein having a tag sequence on a support, the tag sequence including a sequence that includes three or more consecutive basic amino acids.

Claims

1. A method of producing a protein-immobilized support, comprising immobilizing a protein having a tag sequence on a support, wherein the tag sequence comprises a sequence comprising three or more consecutive basic amino acids.

2. The method of producing a protein-immobilized support according to claim 1, wherein the basic amino acid is lysine, arginine, or histidine.

3. The method of producing a protein-immobilized support according to claim 1, wherein the tag sequence is a histidine-tag.

4. The method of producing a protein-immobilized support according to claim 1, wherein the support is magnetic particles.

5. The method of producing a protein-immobilized support according to claim 1, wherein the support has at least one functional group selected from the group consisting of a carboxyl group, an epoxy group, and a tosyl group.

6. A protein-immobilized support obtained by the method of producing a protein-immobilized support according to claim 1.