COMPOSITIONS, METHODS, AND KITS FOR FABRICATING CODED MOLECULAR TAGS

• US 20100105886A1
• Filed: 07/21/2009
• Published: 04/29/2010
• Est. Priority Date: 08/29/2003
• Status: Abandoned Application

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Woudenberg, Timothy M., Sharaf, Muhammad A., Bahatt, Dar, Liu, Timothy Z., Ermakov, Serguei, Connell, Charles R.
• Application Number:
  US12/507,035
• Publication Number:
  US 20100105886A1
• Time in Patent Office: Days
• US Class Current: 536/24.300
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2565/1025 labels being on the same oligonucleotide

Abstract

The present teachings generally relate to probes and probes sets for detecting analytes. The teachings also relates to compositions, methods, and kits for assembling probes comprising at least one coded molecular tag.

Claims

1. A probe comprising at least one reaction portion and at least one identity portion comprising multiple coded molecular tags, wherein the molecular tag is an ordered pattern.

2. The probe of claim 1, further comprising at least one capture ligand.

3. The probe of claim 1, further comprising at least one cross-linker.

4. The probe of claim 3, wherein the cross-linker is cleavable.

5. The probe of claim 1, wherein the coded molecular tag comprises a multiplicity of fluorescent reporter group species.

6. The probe of claim 5, further comprising at least one capture ligand, at least one cleavable cross-linker, or both at least one capture ligand and at least one cleavable cross-linker.

7. A probe set comprising at least one first probe comprising at least one first reaction portion and at least one second separate probe comprising at least one second reaction portion, wherein at least one probe further comprises at least one identity portion comprising multiple coded molecular tags, wherein the molecular tag is an ordered pattern.

8. The probe set of claim 7, wherein at least one probe further comprises at least one analytical portion.

9. The probe set of claim 8, wherein the at least one analytical portion comprises at least one capture ligand, at least one hybridization tag, at least one mobility modifier, at least one aptamer, or combinations thereof.

10. The probe set of claim 9, wherein at least one probe comprises at least identity portion comprising at least one coded molecular tag and at least one corresponding probe comprises at least one reporter group species.

11. The probe set of claim 10, wherein the at least one identity portion further comprises at least one capture ligand.

12. A method for assembling at least one probe comprising, combining at least one coded molecular tag with at least one oligonucleotide comprising at least one reaction portion;

and assembling at least one probe.

13. The method of claim 12, further comprising covalently attaching the at least one coded molecular tag to the at least one oligonucleotide.

14. The method of claim 13, wherein the covalently attaching comprises ligating the at least one probe.

15. The method of claim 14, further comprising at least one ligation template.

16. The method of claim 13, wherein the at least one probe further comprises at least one affinity tag.

17. The method of claim 13, wherein the at least one probe further comprises at least one cross-linker, at least one adapter, or at least one cross-linker and at least one adapter.

18. The method of claim 17, wherein the at least one cross-linker is cleavable.

19. The method of claim 16, wherein the at least one probe further comprises at least one hybridization tag, at least one aptamer sequence, at least one mobility modifier, or combinations thereof.