ILLUMINATION OF DIFFUSELY SCATTERING MEDIA

• US 20100110425A1
• Filed: 03/14/2008
• Published: 05/06/2010
• Est. Priority Date: 03/15/2007
• Status: Active Grant

• Current Assignee: Agilent Technologies Lda Uk Limited (Agilent Technologies, Inc.)
• Original Assignee: Science and Technology Facilities Council
• Inventors: Matousek, Pavel
• Granted Patent: US 8,692,990 B2
• Time in Patent Office: Days
• US Class Current: 356/301
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/44 Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
• CPC Class Codes: G01N 2021/4735 Solid samples, e.g. paper, glass
• CPC Class Codes: G01N 21/47 Scattering, i.e. diffuse reflection G01N21/25, G01N21/41 take precedence G01N21/55 takes precedence
• CPC Class Codes: G01N 21/49 within a body or fluid
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 21/658 enhancement Raman, e.g. surface plasmons

Abstract

The invention provides a technique for increasing the illumination intensity of probe light in a diffusely scattering sample without increasing the power of the probe beam. Generally, an optical filter is used which permits a collimated probe beam of light to pass through to the sample, but which reflects back towards the sample much of the backscattered scattered probe light emerging at a wider range of angles. In particular embodiments a collimated laser beam is delivered to the sample through a multi-layer dielectric filter covering a portion of the sample. The filter is transmissive to the laser light at normal incidence, but reflective at shallower angles of incidence characteristic of the backscattered light.

Claims

1. A method of directing a beam of incident light into a diffusely scattering sample, comprising:

locating a delivery filter adjacent to the sample, the delivery filter having characteristics such that reflection of said incident light is dependent upon angle of incidence of said light at the filter; and

directing a beam of said incident light through the delivery filter at a beam angle of incidence and to the sample,

such that incident light diffusely scattered back out of the sample to arrive at the filter is preferentially reflected by the delivery filter back towards the sample.

2. The method of claim 1 wherein the incident light is of a predetermined wavelength, and the delivery filter is adapted to reflect light of said predetermined wavelength more strongly when incident at a shallower angles of incidence.

3. The method of claim 1 wherein reflection of said incident light by the delivery filter increases at angles of incidence higher than said beam angle.

4. The method of claim 1 wherein the filter characteristics have an incident light transmission feature, which is coincident with the wavelength of the incident light at the beam angle of incidence, and which shifts to shorter wavelengths for increasing angles of incidence.

5. The method of claim 1 wherein the filter characteristics have an incident light reflection feature at longer wavelengths than the wavelength of the incident beam at the beam angle of incidence, and which shifts to shorter wavelengths to cover the beam wavelength at higher angles of incidence.

6. The method of claim 1 wherein the beam at the delivery filter is collimated or semi-collimated.

7. The method of claim 1 wherein the delivery filter is a multi-layer dielectric filter.

8. The method of claim 1 wherein the delivery filter is a holographic filter.

9. The method of claim 1 wherein the delivery filter is spaced from the sample by a distance which is less than a diameter of the incident beam at the sample.

10. The method of claim 1 wherein the delivery filter is spaced from the sample by a distance which is less than a diameter of the delivery filter.

11. The method of claim 1 wherein the delivery filter is spaced from the sample by a distance which is less than a diameter of the sample.

12. The method of claim 1 wherein the delivery filter is curved so as to be adapted to conform to a curved surface of the sample covered by the filter.

13. The method of claim 1 further comprising providing a diffusely scattering spacer element between a surface of the sample to be covered, and the delivery filter.

14. The method of claim 13 wherein the spacer element is deformable so as to adapt to a curved surface of the sample.

15. The method of claim 13 wherein the spacer element is provided with anisotropic scattering characteristics.

16. The method of claim 1 further comprising providing a peripheral mirrored guide surrounding a space between the delivery filter and the surface of the sample covered, so as to retain diffusely scattered light which would otherwise escape around the edge of the covered area.

17. The method of claim 1 further comprising collecting light scattered out of the sample, and analysing said light to detect one or more spectral features of said collected light.

18. The method of claim 17 wherein said one or more spectral features are Raman scattering features.

19. The method of claim 17 wherein the collected light is collected after scattering away from the sample and through the delivery filter.

20. The method of claim 17 wherein the collected light is collected after scattering away from the sample and through a collection filter separate from said delivery filter.

21. An optical enclosure for enhancing the intensity of incident light within a diffusely scattering sample comprising a delivery filter through which a beam of said incident light is directed to the sample at a beam angle of incidence with respect to said filter,

the delivery filter having characteristics such that reflection of said incident light increases at angles of incidence away from the beam angle of incidence,

such that incident light scattered diffusely from the sample is preferentially reflected back into the sample by the delivery filter.

22. The optical enclosure of claim 21 wherein the characteristics of the delivery filter are such that the reflection of said incident light increases at increasing angles of incidence.

23. The optical enclosure of claim 21 wherein the characteristics of said delivery filter include a transmission region which shifts to shorter wavelengths than the incident light for higher angles of incidence.

24. The optical enclosure of claim 21 wherein the delivery filter has a shorter wavelength transmission edge feature, the wavelength of said edge feature shifting to shorter wavelengths with increasing angles of incidence, such that the incident light is transmitted at the beam angle of incidence, and significantly reflected at a range of higher angles of incidence.

25. The optical enclosure of claim 21 wherein the delivery filter is a band pass filter having a band pass wavelength region matched to the wavelength and beam angle of incidence of said beam of incident light.

26. The optical enclosure of claim 21 wherein the delivery filter is a notch filter having a blocking wavelength region matched to block the incident light at a range of beam angles greater than the angle of incidence of said beam of incident light.

27. The optical enclosure of claim 21 wherein said delivery filter is adjacent to said sample.

28. The optical enclosure of claim 27 wherein the delivery filter is spaced from the sample by a distance which is less than a diameter of the sample.

29. The optical enclosure of claim 21 wherein the delivery filter is curved so as to conform to a curved sample surface to be covered by the filter.

30. The optical enclosure of claim 21 further comprising a diffusely scattering spacer element arranged between the delivery filter and a curved surface of the sample.

31. The optical enclosure of claim 30 wherein the spacer element is deformable so as to adapt to a curved surface of the sample.

32. The optical enclosure of claim 30 wherein the spacer element is provided with anisotropic scattering characteristics.

33. The optical enclosure of claim 21 further comprising a peripheral mirrored guide surrounding a space between the delivery filter and the surface of the sample to be covered, so as to retain diffusely scattered light which would otherwise be lost between the sample and the filter.

34. The optical enclosure of claim 21 arranged such that said delivery filter reflects back towards the sample at least 50% of the incident light scattered out of the sample and reaching the filter.

35. The optical enclosure of claim 21 wherein the delivery filter is a dielectric multilayer filter or a holographic filter.

36. The optical enclosure of claim 21 further comprising a collection filter which reflects incident light scattering out of the sample back towards the sample, but transmits spectral features having a different wavelength to said incident light.

37. The optical enclosure of claim 36 wherein the spectral features to be detected are Raman spectral features Stokes shifted to longer wavelengths than the incident light by the diffusely scattering sample.

38. The optical enclosure of claim 36 wherein the collection filter comprises a long wavelength pass filter having an edge lying between the wavelength of the incident light and the spectral features to be detected.

39. The optical enclosure of claim 21 wherein the characteristics of the delivery filter further comprises a transmission feature matched to transmit the spectral features to be detected.

40. The optical enclosure of claim 21 further comprising one or more mirror surfaces arranged across parts of the sample not covered by the delivery filter, to reflect light scattering out of the sample back into the sample.

41. Apparatus comprising the optical enclosure of claim 21, the apparatus being for detecting spectral features of a diffusely scattering sample, the apparatus further comprising:

an incident light source adapted to form said incident light beam;

delivery optics arranged to direct said incident light beam through said delivery filter and to said sample;

collection optics arranged to collect light scattered from said sample; and

a detector arranged to detect one or more spectral characteristics of said collected light.

42. Apparatus for detecting one or more spectral features from a diffusely scattering sample, comprising a delivery filter adapted to allow an incident light beam to pass through at an incident beam angle of incidence to reach the sample, characterised in that the delivery filter is a multi-layer dielectric filter having a transmission characteristic coincident with the wavelength of the incident beam at said angle of incidence, but which shifts to shorter wavelengths at higher angles of incidence such that the filter preferentially reflects back towards the sample incident light diffusely scattered from the sample.

43. The apparatus of claim 42 arranged such that at least 50% of the incident light back scattered from the sample to reach the delivery filter is reflected back towards the sample.

44. The apparatus of claim 42 wherein the delivery filter is positioned within a distance from the sample which is less than a diameter of the filter.

45. The apparatus of claim 42 wherein the incident beam has a beam diameter, and the delivery filter is positioned within a distance from the sample which is less than the incident beam diameter.

46. The apparatus of claim 42 wherein the delivery filter is arranged to cover a region of the sample.

47. A method of illuminating a diffusely scattering sample, comprising:

covering a region of the sample with a delivery filter; and

directing a beam of collimated light of a predetermined wavelength through the delivery filter and into said sample,

wherein said delivery filter is adapted to preferentially reflect back to the sample light of said predetermined wavelength diffusively scattered out of the sample in said region.

48. The method of claim 47 wherein the delivery filter has transmission and/or reflection characteristics which shift to shorter wavelengths at shallower angles of incidence.

49. The method of claim 47 wherein the delivery filter characteristics have a transmission region coincident with the predetermined wavelength at a first range of angles of incidence, and is shifted away from the predetermined wavelength at a second range of angles of incidence.

50. The method of claim 47 wherein the delivery filter characteristics have a transmission region coincident with the predetermined wavelength at substantially normal angles of incidence.

51. A method of collecting spectrally changed light from a diffusely scattering sample illuminated with light of an incident wavelength, comprising:

covering a region of the sample with a collection filter; and

collecting said spectrally changed light through the collection filter,

wherein said collection filter is adapted to reflect back to the sample light of said incident wavelength diffusively scattered out of the sample in said region, and to preferentially allow said spectrally changed light to pass.