SYSTEMS AND DEVICES FOR SEQUENCE BY SYNTHESIS ANALYSIS
First Claim
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1. A system for sequencing one or more polynucleotide, the system comprising:
- a) a solid substrate comprising one or more polynucleotides attached thereto;
b) a fluid direction system for controllably moving one or more fluorescently labeled reagents into contact with the polynucleotides;
c) a temperature control system for regulating the temperature of at least one of the substrate and the reagents;
d) an illumination system for exciting the fluorescent label via total internal reflection (TIR);
e) a detector component proximal to the substrate for detecting fluorescence produced from excitation of the fluorescent label by the TIR system;
f) a computer system operably coupled to the detector wherein the computer comprises an instruction set for acquiring fluorescence images from the detector.
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Abstract
The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.
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33 Claims
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1. A system for sequencing one or more polynucleotide, the system comprising:
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a) a solid substrate comprising one or more polynucleotides attached thereto; b) a fluid direction system for controllably moving one or more fluorescently labeled reagents into contact with the polynucleotides; c) a temperature control system for regulating the temperature of at least one of the substrate and the reagents; d) an illumination system for exciting the fluorescent label via total internal reflection (TIR); e) a detector component proximal to the substrate for detecting fluorescence produced from excitation of the fluorescent label by the TIR system; f) a computer system operably coupled to the detector wherein the computer comprises an instruction set for acquiring fluorescence images from the detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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Specification