FOCUSING CHAMBER
First Claim
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1. A method positioning agents in a fluid flow, the method comprising:
- providing a chamber having a substrate;
flowing a first fluid containing agents through a first fluid port and through the substrate in the chamber;
flowing a second fluid flow through one or more second fluid ports positioned about the substrate in the chamber, the second fluid flow at least partially surrounding the first fluid flow to direct the first fluid flow and any agents contained therein toward a central portion of the substrate;
positioning the agents on the central portion of the substrate;
reversing flow through the first fluid port to move any agents positioned on the substrate out of the chamber in central streamlines that exit the chamber through the first fluid port; and
removing flow from about the central streamlines to separate fluid flow that lacks agents from the central streamlines that exit the chamber.
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Abstract
Aspects of the invention relate to devices and methods of use thereof for concentrating, positioning and/or manipulating agents within a fluid, including but not limited to genomic DNA.
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Citations
25 Claims
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1. A method positioning agents in a fluid flow, the method comprising:
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providing a chamber having a substrate; flowing a first fluid containing agents through a first fluid port and through the substrate in the chamber; flowing a second fluid flow through one or more second fluid ports positioned about the substrate in the chamber, the second fluid flow at least partially surrounding the first fluid flow to direct the first fluid flow and any agents contained therein toward a central portion of the substrate; positioning the agents on the central portion of the substrate; reversing flow through the first fluid port to move any agents positioned on the substrate out of the chamber in central streamlines that exit the chamber through the first fluid port; and removing flow from about the central streamlines to separate fluid flow that lacks agents from the central streamlines that exit the chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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- 22. The method of claim 21, wherein agents are conveyed without dispersing more than 2 cm in a direction of flow.
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22-1. A composition comprising isolated nucleic acids varying in length from 0.1 megabases to about 1 megabase in a fluid having a volume between about 30 uL to about 40 uL.
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25. A method positioning agents in a fluid flow, the method comprising:
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providing a substrate; flowing a first fluid containing agents through the substrate; flowing a second fluid flow through the substrate, the second fluid flow at least partially surrounding the first fluid flow to direct the first fluid flow and any agents contained therein toward a central portion of the substrate; positioning the agents on the central portion of the substrate; and reversing flow through substrate to move any agents positioned on the substrate away from the substrate in central streamlines of fluid moving away from the substrate.
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Specification