Differentiation of Human Embryonic Stem Cells
First Claim
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1. A method of increasing the expression of markers associated with the pancreatic endocrine lineage comprising treating cells expressing markers associated with the pancreatic endocrine lineage with medium comprising a sufficient amount of a TGF-β
- receptor agonist to cause an increase in expression of markers associated with the pancreatic endocrine lineage.
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Abstract
The present invention provides methods to promote the differentiation of pluripotent stem cells. In particular, the present invention provides a method to increase the expression of markers associated with the pancreatic endocrine lineage.
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Citations
7 Claims
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1. A method of increasing the expression of markers associated with the pancreatic endocrine lineage comprising treating cells expressing markers associated with the pancreatic endocrine lineage with medium comprising a sufficient amount of a TGF-β
- receptor agonist to cause an increase in expression of markers associated with the pancreatic endocrine lineage.
- View Dependent Claims (2, 3)
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4. A method for differentiating pluripotent stem cells, comprising the steps of:
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a. Culturing the pluripotent stem cells, b. Differentiating the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage, c. Differentiating the cells expressing markers characteristic of the definitive endoderm lineage into cells expressing markers characteristic of the pancreatic endoderm lineage, and d. Differentiating the cells expressing markers characteristic of the pancreatic endoderm lineage into cells expressing markers characteristic of the pancreatic endocrine lineage. - View Dependent Claims (5, 6, 7)
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