Apparatus And Methods For Processing Tissue to Release Cells

• US 20100112695A1
• Filed: 11/03/2008
• Published: 05/06/2010
• Est. Priority Date: 11/03/2008
• Status: Abandoned Application

• Current Assignee: Baxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
• Original Assignee: Baxter Healthcare SA (Baxter International Inc.), Baxter International Inc.
• Inventors: Min, Kyungyoon
• Application Number:
  US12/263,984
• Publication Number:
  US 20100112695A1
• Time in Patent Office: Days
• US Class Current: 435/381
• CPC Class Codes: C12M 45/02 by mechanical forces; Stirring; Trituration; Comminuting crushing, pulverizing, disintegrating in general B02C
• CPC Class Codes: C12M 47/04 Cell isolation or sorting purging biological preparations of unwanted cells C12N5/0081, determining the presence or kind of microorganism C12Q1/04

Abstract

An apparatus and methods for processing tissue to release biological material including cells are disclosed.

Claims

1-47. -47. (canceled)

48. An apparatus for processing tissue to release cells from the tissue, comprising:

a first housing having a porous wall portion, the first housing adapted to receive a tissue sample and the wall portion being sufficiently porous to allow passage therethrough of material including cells derived from the tissue;

a second housing that at least substantially encloses the first housing;

at least one of the first and second housings being movable to assist in processing of tissue in the first housing and passage of material including cells derived from the tissue through the porous wall portion of the first housing; and

an agitator disposed within the first housing and movable relative to the first housing and second housing to enhance tissue processing therein.

49. The apparatus of claim 48 wherein the agitator includes a spiral agitation surface.

50. The apparatus of claim 48 in which the agitator is rotatable relatively to the first housing, the first housing has an axis and rotation of the agitator induces flow in an axial direction.

51. The apparatus of claim 50 in which rotation of the agitator also induces flow in a radial direction.

52. The apparatus of claim 48 wherein the agitator extends through substantially the full length of the first housing.

53. The apparatus of claim 48 wherein the agitator comprises an augur.

54. The apparatus of claim 48 wherein the agitator includes a drive shaft and the apparatus includes a motor external to the first housing operatively associated with the drive shaft.

55. The apparatus of claim 48 wherein the agitator includes a magnetic drive member.

56. The apparatus of claim 48 wherein the agitator is rotatable relative to the first housing and the first housing is movable through rotation and is rotatable in a direction opposite to the direction of rotation of the agitator.

57. The apparatus of claim 48 wherein the agitator is rotatable relative to the first housing and is configured such that upon rotation it creates shear forces on tissue within the first housing.

58. The apparatus of claim 48 wherein the agitator is configured for the processing of adipose tissue to release stem cells.

59. A method of using an apparatus for processing tissue to release cells from the tissue, comprising:

a. inserting a tissue sample containing cells into a first housing wherein the first housing has a porous wall portion, the first housing adapted to receive a tissue sample and the wall portion being sufficiently porous to allow passage therethrough of material including cells derived from the tissue, a second housing that at least substantially encloses the first housing and an agitator disposed within the first housing;

b. introducing at least one tissue-releasing agent into at least one of the housings; and

c. moving at least one of the first housing and the second housing and moving the agitator relative to the to process the tissue sample and to pass material including cells derived from the tissue sample through the porous outer wall of the first housing.

60. The method of claim 59 wherein the agitator includes a spiral agitation surface.

61. The method of claim 59 in which the agitator is rotatable relative to the first housing and the first housing has an axis and the rotation of the agitator induces flow in an axial direction.

62. The method of claim 61 in which rotation of the agitator also induces flow in a radial direction.

63. The method of claim 59 wherein the agitator comprises an auger.

64. The method of claim 59 wherein the agitator extends through substantially the full length of the first housing.

65. The method of claim 59 wherein the agitator includes a drive shaft and the apparatus includes a motor external to the first housing operatively associated with the drive shaft.

66. The method of claim 59 wherein the agitator is moved by a magnetic drive member.

67. The method of claim 59 wherein the agitator is rotatable relative to the first housing and the first housing is movable through rotation and is rotatable in a direction opposite to the direction of rotation of the agitator.

68. The method of claim 59 wherein the agitator is rotatable relative to the first housing and the agitator is configured such that upon rotation, it creates shear effects on tissue within the first housing.

69. The method of claim 59 wherein the tissue comprises adipose tissue.