Polypeptide Display Libraries and Methods of Making and Using Thereof
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Abstract
Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed.
37 Citations
43 Claims
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1-20. -20. (canceled)
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21. A composition comprising:
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a replicable biological entity; and a passenger polypeptide displayed on the surface of the replicable biological entity, wherein the passenger polypeptide is fused to an extracellular terminus of a circularly permuted bacterial outer membrane protein. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A polypeptide display library comprising:
a plurality of replicable biological entities, each member of the plurality comprising a passenger polypeptide displayed on the surface of the member replicable biological entity, wherein the passenger polypeptide is fused to an extracellular terminus of a circularly permuted bacterial outer membrane protein. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43)
Specification