Sensor

• US 20100116035A1
• Filed: 04/30/2008
• Published: 05/13/2010
• Est. Priority Date: 04/30/2007
• Status: Active Grant

• Current Assignee: Attana AB
• Original Assignee: Attana AB
• Inventors: Aastrup, Teodor, Anderson, Henrik, Björkman, Henrik
• Granted Patent: US 8,196,455 B2
• Time in Patent Office: Days
• US Class Current: 73/61.490
• CPC Class Codes: G01N 2291/0255 (Bio)chemical reactions, e.g. on biosensors
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0426 Bulk waves, e.g. quartz crystal microbalance, torsional waves
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B
• CPC Class Codes: G01N 29/222 Constructional or flow details for analysing fluids optoacoustic fluid cells G01N29/2425

Abstract

A sensor element carrier for use in a mass-sensitive chemical sensor instrument comprises a base component and a lid component, the base component being adapted to receive, in use, a sensor, the signal generated by which depends on the mass of material adsorbed at a sensing surface thereof, the base component or the lid component having formed therein, in a recessed area, at least one channel for the ingress of sample fluid, the recessed area forming, in use and in conjunction with the sensing surface of the sensor, a sample chamber, the sensor being held, in use, between the base component and the lid component and the approach of the base component and lid component being limited on assembly of the sensor element by means of substantially rigid portions of each which come into abutment, thereby limiting the minimum height of the sample chamber for a given set of sensor, base and lid components.

Claims

1. A sensor element carrier for use in a mass-sensitive chemical sensor instrument, comprising:

(a) a base component;

(b) a lid component;

(c) a sample chamber formed from a recess area of the base component or the lid component and, when in use, comprising a sensor having a sensing surface, a signal generated by which depends on the mass of material adsorbed at the sensing surface thereof; and

(d) at least one channel-for the ingress of a sample fluid, in the-recessed area, and wherein the base component and the lid component having substantially rigid portions are limited on assembly of the sensor element, when in use, by the substantially rigid portions of each which come into abutment, thereby limiting the minimum height of the sample chamber for a given set of sensor, base and lid components.

2. The sensor element carrier according to claim 1, further comprising a compressible sealing member surrounding the recessed area, wherein the compressible sealing member protrudes beyond the recessed area and beyond the non-recessed portion of the base or lid component surrounding the recessed area, such that the compressible sealing member abuts, in use, the surface of the sensor so as to form a plurality of lateral edges of the sample chamber.

3. The sensor element carrier according to claim 2 wherein the compressible sealing member is an elastomeric ring having a cross-sectional shape which is capable of deformation, upon application of axial force, by bending.

4. The sensor element carrier according to claim 3 wherein the compressible sealing member is an elastomeric ring having an ‘

X’

-shaped, or equivalent, cross section.

5. The sensor element carrier according to claim 4 wherein, in use, the elastomeric ring and the sensor are arranged such that only the inner protruding leg of the ‘

X’

-shaped, or equivalent, cross section contacts the sensor and is less compressed than an outer protruding leg, wherein the outer protruding leg contacts the base component or lid component.

6. The sensor element carrier according to claim 1, wherein the recessed area having the at least one channel is formed in the lid component.

7. The sensor element carrier according to claim 1, wherein the recessed area has a first channel formed therein for the ingress of sample fluid, and a second channel formed therein for the egress of sample fluid, such that the sample chamber can be used as a flow cell.

8. The sensor element carrier according to claim 1, further comprising at least two carrier electrodes for connecting to a driving and counter electrodes.

11. The sensor element carrier according to claim 1, wherein the base component or lid component has a cavity adjacent the non-sensing surface of the sensor, when in use, to avoid damping of the piezoelectric resonance of a sensor based on a quartz crystal microbalance.

12. The sensor element carrier according to claim 1, wherein an aperture is formed in the base component or lid component, such that, when in use, the non-sensing surface of a sensor based on evanescent wave sensing is optically accessed.

14. The sensor element carrier according to claim 1, wherein the sensor element carrier is fitted with a sealable optical window, such that, when in use, the sensing surface of a sensor is interrogated via the optical window.

15. The sensor element carrier according to claim 1, wherein the lid component of the sensor element carrier comprises a first part and a second part, wherein the first part is being adapted for assembly with the base component and has an aperture which, when in use, is in registration with a sensor mounted in the carrier, and wherein the second part comprises a plate having a protruding portion shaped such that, on assembly of the sensor element, the protruding portion passes through the aperture to form the recessed area which, when in use, and in conjunction with the sensing surface of the sensor, forms the sample chamber.

27. The carrier according to claim 1, wherein the lid component and base component are formed from a material selected from the group consisting of polyoxymethylene, polymethylmethacrylate, polyvinyl chloride and injection-moldable thermoplastics, polystyrene, acrylonitrile-butadiene-styrene, and composites thereof.

34. The sensor element carrier according to claim 1, wherein the sensing surface of the sensor is at least partially covered with a polymeric coating or a self-assembled monolayer.

9-10. -10. (canceled)

13. (canceled)

16. A sensor element for use in a mass-sensitive chemical sensor instrument, comprising a mass-sensitive sensor having a sensing surface and a carrier wherein the carrier comprises:

(a) a base component;

(b) a lid component;

(c) a sample chamber formed from a recess area of the base component or the lid component; and

(d) at least one channel-for the ingress of a sample fluid in the recess area, and wherein base component and the lid component have substantially rigid portions are limited on assembly of the sensor element, when in use, by the substantially rigid portions of each which come into abutment, thereby limiting the minimum height of the sample chamber for a given set of sensor, base and lid components.

17. The sensor element according to claim 16, wherein the sensor is a quartz crystal microbalance, wherein, when assembled, the quartz crystal microbalance is sealed from the external environment, except for the channels for the ingress and egress of sample fluid.

18. The sensor element according to claim 16, wherein the sensor is an evanescent wave-based device and the base or lid component of the sensor element carrier has an aperture formed therein for optical access to the non-sensing surface of the sensor.

20. The sensor element according to claim 17 wherein the carrier comprises two electrodes for connection to the driving and counter electrodes of the quartz crystal microbalance, the carrier electrodes being accessible from the exterior of the carrier.

21. The sensor element according to claim 20 wherein the carrier electrodes are accessible via apertures in the base component or lid component.

22. The sensor element according to claim 17, wherein the quartz crystal microbalance comprises a quartz crystal plate having a first face and a second face, and provided with a driving electrode having an exposed surface on the first face of the quartz crystal plate and a counter electrode having an exposed surface on the second face of the quartz crystal plate, wherein the exposed surface area of each of the driving electrode and counter electrode is smaller than that of the first and second crystal faces on which it is provided, whereby a peripheral region of the crystal is not provided with electrode material, and whereby each of the driving and counter electrodes has a connecting portion extending toward a periphery of the quartz crystal plate.

23. The sensor element according to claim 22 wherein the connecting portion of the driving or counter electrode extends to the periphery of the first face of the quartz crystal plate and round to the second face thereof, without contacting the counter or driving electrode, respectively, provided on the second face of the quartz crystal plate, whereby both the driving and counter electrodes are contacted from one face of the quartz crystal plate.

24. The sensor element according to claim 22 wherein both driving and counter electrodes are contacted by carrier electrodes located in the base component.

26. The sensor element according to claim 16, wherein the sensing surface of the sensor is at least partially covered with a polymeric coating or a self-assembled monolayer.

33. The sensor element according to claim 16, wherein the lid component and base component are formed from a material selected from the group consisting of polyoxymethylene, polymethylmethacrylate, polyvinyl chloride and injection-moldable thermoplastics, polystyrene, acrylonitrile-butadiene-styrene, and composites thereof.

19. (canceled)

25. (canceled)

28. A mass-sensitive chemical sensor instrument, comprising a sensor element, wherein the sensor element comprises a mass-sensitive sensor having a sensing surface and a carrier wherein the carrier comprises:

(a) a base component;

(b) a lid component;

(c)) a sample chamber formed from a recess area of the base component or the lid component and comprising, when in use, the sensor element; and

(d) at least one channel-for the ingress of a sample fluid, and wherein base component and the lid component have substantially rigid portions are limited on assembly of the sensor element, when in use, by the substantially rigid portions of each which come into abutment, thereby limiting the minimum height of the sample chamber for a given set of sensor, base and lid components.

29. (canceled)

30. A method for mass-sensitive chemical sensing, comprising:

(1) introducing a sample fluid through an ingress channel of a sensor element, wherein the sensor element comprises a mass-sensitive sensor and a carrier comprising;

(a) a base component wherein the base component is adapted to receive a lid component in use;

(b) a lid component;

(c) a sample chamber formed from a recess area of the base component or the lid component,

(d) at least one channel for the ingress of a sample fluid, and wherein base component and the lid component have substantially rigid portions are limited on assembly of the sensor element, when in use, by the substantially rigid portions of each which come into abutment, thereby limiting the minimum height of the sample chamber for a given set of sensor, base and lid components;

(2) allowing chemical species within the sample fluid to interact with the sensor; and

(3) sensing, by means of a change in signal generated by the sensor, such interaction.

31. A sensor element carrier for use in a mass-sensitive chemical sensor instrument, the sensor element carrier comprising a base component and a lid component, the base component being adapted to receive, in use, a sensor, the signal generated by which depends on the mass of material adsorbed at a sensing surface thereof, the base component or the lid component having formed therein, in a recessed area, at least one channel for the ingress of sample fluid, the recessed area forming, in use and in conjunction with the sensing surface of the sensor, a sample chamber, the sensor being held, in use, between the base component and the lid component, wherein the lid and/or base component of the sensor element carrier comprises first and second parts, the first part being adapted for assembly with the other of the base or lid components and having an aperture which, in use, is in registration with a sensor mounted in the carrier, and the second part comprising a plate having a protruding portion shaped such that, on assembly of the sensor element, the protruding portion passes through the aperture to form the recessed area which, in use, and in conjunction with the sensing surface of the sensor, forms the sample chamber.

32. A sensor element carrier according to claim 31, wherein the first part of the lid component or first part of the base component, or the corresponding base or lid component for assembly therewith, is adapted such that, when the base or lid component and the first part of the lid component or first part of the base component, respectively, are assembled in use with a sensor mounted in the base component, the sensor is sealed between the base component and the first part of the lid component, or between the lid component and first part of the base component, respectively, so as to prevent fluid placed onto the sensor via the aperture in the first part of the lid component or first part of the base component from escaping, other than by means of the said aperture.