METHODS AND SYSTEMS FOR DETECTION OF CONTAMINANTS

• US 20100120016A1
• Filed: 08/31/2007
• Published: 05/13/2010
• Est. Priority Date: 09/01/2006
• Status: Abandoned Application

• Current Assignee: The Board of Trustees of the University of Arkansas (University of Arkansas System)
• Original Assignee: Texas A&M University System
• Inventors: Berghman, Luc, Srinivasan, Balaji, Tung, Steve, Ye, Zunzhong, Wang, Ronghui, Bottje, Walter, Hargis, Billy, Li, Yanbin, Varshney, Madhukar
• Application Number:
  US12/439,462
• Publication Number:
  US 20100120016A1
• Time in Patent Office: Days
• US Class Current: 435/5
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: C12Q 1/06 Quantitative determination

Abstract

An impedance biosensor for detecting a contaminant in a starting material, the biosensor comprising a housing, an input device supported by the housing, an output device supported by the housing, a microfluidic cell supported by the housing, the starting material being engagable with the microfluidic cell, and an impedance analyzer supported by the housing and operable to measure impedance of the starting material to detect the presence of a contaminant.

Claims

1-52. -52. (canceled)

53. A method of detecting a virus in a starting material comprising:

a) contacting the starting material with a red blood cell, wherein the virus is capable of binding to the red blood cell to form a complex; and

b) detecting the complex with a biosensor, wherein detection of the complex is indicative of the presence of the virus in the starting material.

54. The method of claim 53, wherein step b) further comprises determining the quantity of the virus in the starting material.

55. The method of claim 53, wherein the starting material is selected from the group consisting of a food product, an animal-derived sample, an environmental sample, and a clinical sample.

56. The method of claim 53, wherein the virus is selected from the group consisting of influenza, avian influenza H5N1, infectious bronchitis virus and Newcastle virus.

57. The method of claim 53, wherein step a) further comprises contacting the starting material with an affinity moiety capable of binding to the virus, wherein the affinity moiety is coupled to a magnetic nanoparticle, to form a magnetic target.

58. The method of claim 57, wherein the affinity moiety is an antibody.

59. The method of claim 57, wherein step a) further comprises separating the magnetic target from the starting material.

60. The method of claim 57, wherein the affinity moiety is not attached to the biosensor.

61. The method of claim 57, wherein step b) further comprises

delivering the magnetic target to the biosensor, wherein the biosensor comprises a microfluidic cell having an interdigitated array microelectrode disposed therein;

and

detecting the magnetic target with the interdigitated array microelectrode, wherein detection of the target is indicative of the presence of the contaminant in the starting material.

62. The method of claim 61, wherein step b) further comprises measuring impedance of the interdigitated array microelectrode.

63. The method of claim 61, wherein step b) further comprises determining the quantity of the virus in the starting material.

64. The method of claim 61, wherein the starting material is selected from the group consisting of a food product, an animal-derived sample, an environmental sample, and a clinical sample.

65. The method of claim 61, wherein the virus is selected from the group consisting of influenza, avian influenza H5N1, infectious bronchitis virus and Newcastle virus.